Zhao Wei, Liu Jun, Qian Lunwen, Guan Mei, Guan Chunyun
College of Agronomy, Hunan Agricultural University, Changsha 410128, China.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
Plants (Basel). 2022 Aug 29;11(17):2241. doi: 10.3390/plants11172241.
Oil-body-membrane proteins (OBMPs) are essential structural molecules of oil bodies and also versatile metabolic enzymes involved in multiple cellular processes such as lipid metabolism, hormone signaling and stress responses. However, the global landscape for genes in oil crops is still lacking. Here, we performed genome-wide identification and characterization of genes in polyploid crop . contains up to 88 genes including 53 oleosins, 20 caleosins and 15 steroleosins. Both whole-genome and tandem duplications have contributed to the expansion of the gene family. These genes have extensive sequence polymorphisms, and some harbor strong selection signatures. Various cis-acting regulatory elements involved in plant growth, phytohormones and abiotic and biotic stress responses are detected in their promoters. exhibit differential expression at various developmental stages from diverse tissues. Importantly, some genes display spatiotemporal patterns of seed-specific expression, which could be orchestrated by transcriptional factors such as EEL, GATA3, HAT2, SMZ, DOF5.6 and APL. Altogether, our data lay the foundations for studying the regulatory mechanism of the seed oil storage process and provide candidate genes and alleles for the genetic improvement and breeding of rapeseed with high seed oil content.
油体膜蛋白（OBMPs）是油体的重要结构分子，也是参与脂质代谢、激素信号传导和应激反应等多种细胞过程的多功能代谢酶。然而，目前仍缺乏油料作物中该基因的整体情况。在此，我们对多倍体作物中的该基因进行了全基因组鉴定和特征分析。该作物包含多达88个该基因，其中包括53个油质蛋白、20个钙结合油体蛋白和15个固醇油体蛋白。全基因组重复和串联重复都对该基因家族的扩张有所贡献。这些基因具有广泛的序列多态性，有些还带有强烈的选择印记。在它们的启动子中检测到了参与植物生长、植物激素以及非生物和生物胁迫反应的各种顺式作用调控元件。它们在不同组织发育阶段表现出差异表达。重要的是，一些基因呈现种子特异性表达的时空模式，这可能由EEL、GATA3、HAT2、SMZ、DOF5.6和APL等转录因子调控。总之，我们的数据为研究种子油脂储存过程的调控机制奠定了基础，并为高种子油含量油菜籽的遗传改良和育种提供了候选基因和等位基因。
