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生物强化旋转生物接触器降解 2-氟苯酚。

Bioaugmentation of a rotating biological contactor for degradation of 2-fluorophenol.

机构信息

CBQF/Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal.

出版信息

Bioresour Technol. 2011 Oct;102(19):9300-3. doi: 10.1016/j.biortech.2011.07.003. Epub 2011 Jul 14.

Abstract

The performance of a laboratory scale rotating biological contactor (RBC) towards shock loadings of 2-fluorophenol (2-FP) was investigated. During a period of ca. 2 months organic shock loadings of 25 mg L⁻¹ of 2-FP were applied to the RBC. As no biodegradation of 2-FP was observed, bioaugmentation of the RBC with a 2-FP degrading strain was carried out and, along ca. 6 months, organic shock loadings within a range of 25-200 mg L⁻¹ of 2-FP were applied. Complete biodegradation of 50 mg L⁻¹ of 2-FP was observed during operation of the reactor. The RBC showed to be robust towards starvation periods, as after ca. 1month of non-supply of the target compound, the reactor resumed 2-FP degradation. The inoculated strain was retained within the biofilm in the disks, as the 2-FP degrading strain was recovered from the biofilm by the end of the experiment, thus bioaugmentation was successfully achieved.

摘要

研究了实验室规模的旋转生物接触器(RBC)对 2-氟苯酚(2-FP)冲击负荷的性能。在大约 2 个月的时间里,向 RBC 中施加了约 25mg/L 的 2-FP 有机冲击负荷。由于未观察到 2-FP 的生物降解,因此对 RBC 进行了 2-FP 降解菌的生物增强,并且在大约 6 个月的时间里,施加了 25-200mg/L 的 2-FP 有机冲击负荷。在反应器的运行过程中,观察到 50mg/L 的 2-FP 完全生物降解。RBC 表现出对饥饿期的稳健性,因为在大约 1 个月的时间内未供应目标化合物后,反应器恢复了 2-FP 的降解。接种的菌株保留在圆盘的生物膜内,因为到实验结束时,从生物膜中回收了 2-FP 降解菌,因此成功地实现了生物增强。

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