Torsvik V, Salte K, Sørheim R, Goksøyr J
Department of Microbiology and Plant Physiology, University of Bergen, Norway.
Appl Environ Microbiol. 1990 Mar;56(3):776-81. doi: 10.1128/aem.56.3.776-781.1990.
The phenotypic diversity of about 200 bacterial strains isolated from soil was compared with the genotypic diversity of the same population. The strains were phenotypically characterized by the API 20B test system. The results of these tests were subjected to cluster analysis, which revealed 41 biotypes at 80% similarity. The five dominating biotypes contained 43% of the strains. The phenotypic diversity as determined by the Shannon index, equitability, rarefaction, and cumulative differences was high, but indicated some dominant biotypes. The genetic diversity was measured by reassociation of mixtures of denatured DNA isolated from the bacterial strains (C0t plots). The observed genetic diversity was high. Reassociation of DNA from all bacterial strains together revealed that the population contained heterologous DNA equivalent to 20 totally different bacterial genomes (i.e., genomes that have no homology). This study showed that reassociation of DNA isolated from a collection of bacteria gave a good estimate of the diversity of the collection and that there was good agreement with different phenotypic diversity measures. The Shannon index in particular has features in common with the genetic diversity measure presented here.
将从土壤中分离出的约200株细菌菌株的表型多样性与同一群体的基因型多样性进行了比较。这些菌株通过API 20B测试系统进行表型特征分析。对这些测试结果进行聚类分析，结果显示在80%相似度水平上有41种生物型。五种主要生物型包含了43%的菌株。通过香农指数、公平性、稀疏化和累积差异确定的表型多样性较高，但也表明存在一些优势生物型。通过对从细菌菌株中分离出的变性DNA混合物进行重新结合（Cot曲线）来测量遗传多样性。观察到的遗传多样性较高。将所有细菌菌株的DNA一起重新结合表明，该群体包含相当于20个完全不同细菌基因组（即无同源性的基因组）的异源DNA。这项研究表明，从一组细菌中分离出的DNA重新结合能很好地估计该组的多样性，并且与不同的表型多样性测量方法有很好的一致性。特别是香农指数与这里提出的遗传多样性测量方法有共同特征。
