中华刺参叶中成分的化学结构及保肝作用

Chemical structures and hepatoprotective effects of constituents from the leaves of Salacia chinensis.

作者信息

Nakamura Seikou, Zhang Yi, Matsuda Hisashi, Ninomiya Kiyofumi, Muraoka Osamu, Yoshikawa Masayuki

机构信息

Kyoto Pharmaceutical University, Kyoto, Japan.

出版信息

Chem Pharm Bull (Tokyo). 2011;59(8):1020-8. doi: 10.1248/cpb.59.1020.

DOI:10.1248/cpb.59.1020

PMID:21804248

Abstract

The methanolic extract from the leaves of Salacia chinensis collected in Thailand was found to show a protective effect on D-galactosamine-induced cytotoxicity in primary cultured mouse hepatocytes. From the methanolic extract, eight new glycosides, named foliachinenosides E, F, G, H, and I, and foliasalaciosides J, K and L, were isolated together with 26 known constituents. The structures of new glycosides were determined on the basis of physicochemical and chemical evidence. In addition, the hepatoprotective effects of the isolated compounds on D-galactosamine-induced cytotoxicity were examined. Among them, lignans, eleutheroside E₂ and 7R,8S-dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside, were found to show the protective effects [inhibition (%) 41.4 ± 3.6 (p < 0.01), 45.5 ± 2.7 (p < 0.01) at 100 µM, respectively].

摘要

研究发现，采自泰国的中华五层龙叶片的甲醇提取物对原代培养的小鼠肝细胞中D - 半乳糖胺诱导的细胞毒性具有保护作用。从该甲醇提取物中分离出8种新的糖苷，分别命名为五层龙苷E、F、G、H和I，以及五层龙糖苷J、K和L，同时还分离出26种已知成分。根据物理化学和化学证据确定了新糖苷的结构。此外，还检测了分离出的化合物对D - 半乳糖胺诱导的细胞毒性的肝保护作用。其中，木脂素、刺五加苷E₂和7R,8S - 二氢脱氢二松柏醇4 - O - β - D - 吡喃葡萄糖苷在100 μM时显示出保护作用[抑制率(%)分别为41.4 ± 3.6 (p < 0.01)、45.5 ± 2.7 (p < 0.01)]。