ALDH1 在宫颈癌中的表达。

The expression of ALDH1 in cervical carcinoma.

机构信息

Department of Gynecological Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

出版信息

Med Sci Monit. 2011 Aug;17(8):HY21-26. doi: 10.12659/msm.881886.

BACKGROUND

ALDH1 has been shown to play a role in the early differentiation of stem cells in some human malignancies. Whether cancer stem cells occur in ALDH1-associated cervical cancer is not known.

MATERIAL/METHODS: We tested the hypothesis that cervical carcinomas contain subpopulations of cells that express ALDH1.The following sources of cervical carcinoma tissues were examined for the presence of stem cell marker ALDH1 by immunohistochemistry. Flow cytometric isolation of cancer cells was based on enzymatic activity of ALDH (Aldefluor assay). The mRNA and protein levels of ALDH1 were investigated by qRT-PCR and Western blot, respectively. We also detected the expression of CD133 identified as a stem cell marker for several cancers.

RESULTS

23/89 samples of invasive squamous carcinoma and 4/20 samples of adenocarcinomas exhibited immunoreactivity to stem cell marker aldehyde dehydrogenase 1 (ALDH1). Expression of ALDH1 was found in 24.77% of the samples. Flow cytometric analysis, qRT-PCR and Western blot also confirmed the presence of small subpopulations of ALDH1-positive cells. In contrast, we found cervical carcinoma had low CD133 population.

CONCLUSIONS

Cervical carcinoma contains a small subpopulation of cells that may relate to a cancer stem cell-like phenotype, ALDH1.

背景

ALDH1 已被证明在一些人类恶性肿瘤的干细胞早期分化中发挥作用。是否在与 ALDH1 相关的宫颈癌中存在癌症干细胞尚不清楚。

材料/方法：我们通过免疫组织化学检测假设宫颈癌中存在表达 ALDH1 的细胞亚群。通过流式细胞术基于 ALDH 的酶活性（Aldefluor 测定法）分离癌细胞。通过 qRT-PCR 和 Western blot 分别检测 ALDH1 的 mRNA 和蛋白水平。我们还检测了作为几种癌症的干细胞标志物的 CD133 的表达。

结果

23/89 例浸润性鳞状细胞癌和 4/20 例腺癌样本对干细胞标志物醛脱氢酶 1（ALDH1）表现出免疫反应性。在 24.77%的样本中发现了 ALDH1 的表达。流式细胞术分析、qRT-PCR 和 Western blot 也证实了存在少量 ALDH1 阳性细胞亚群。相比之下，我们发现宫颈癌中 CD133 群体较少。