I-Shou University, Department of Biomedical Engineering, Kaohsiung, Taiwan.
J Biomed Opt. 2011 Jul;16(7):076006. doi: 10.1117/1.3595860.
Optoelectronics techniques to induce protoporphyrin IX fluorescence with topically applied 5-aminolevulinic acid on the oral mucosa have been developed to noninvasively detect oral cancer. Fluorescence imaging enables wide-area screening for oral premalignancy, but the lack of an adequate fluorescence enhancement method restricts the clinical imaging application of these techniques. This study aimed to develop a reliable fluorescence enhancement method to improve PpIX fluorescence imaging systems for oral cancer detection. Three contrast features, red-green-blue reflectance difference, R∕B ratio, and R∕G ratio, were developed first based on the optical properties of the fluorescence images. A comparative study was then carried out with one negative control and four biopsy confirmed clinical cases to validate the optimal image processing method for the detection of the distribution of malignancy. The results showed the superiority of the R∕G ratio in terms of yielding a better contrast between normal and neoplastic tissue, and this method was less prone to errors in detection. Quantitative comparison with the clinical diagnoses in the four neoplastic cases showed that the regions of premalignancy obtained using the proposed method accorded with the expert's determination, suggesting the potential clinical application of this method for the detection of oral cancer.
已经开发出光电技术，通过局部应用 5-氨基酮戊酸在口腔黏膜上诱导原卟啉 IX 荧光，以非侵入性方式检测口腔癌。荧光成像是广泛筛查口腔癌前病变的手段，但缺乏有效的荧光增强方法限制了这些技术在临床成像中的应用。本研究旨在开发一种可靠的荧光增强方法，以改善用于口腔癌检测的 PpIX 荧光成像系统。首先，根据荧光图像的光学特性，开发了三种对比特征，即红-绿-蓝反射率差异、R/B 比值和 R/G 比值。然后，对一个阴性对照和四个活检证实的临床病例进行了对比研究，以验证最佳图像处理方法在检测恶性分布方面的效果。结果表明，R/G 比值在正常组织和肿瘤组织之间产生更好对比度方面具有优越性，并且这种方法在检测中不易出错。与四个肿瘤病例的临床诊断进行定量比较表明，使用所提出的方法获得的癌前病变区域与专家的判断一致，表明该方法在检测口腔癌方面具有潜在的临床应用价值。
