Yusoff Nur Afizah, Taib Izatus Shima, Budin Siti Balkis, Mohamed Mahaneem
Biomedical Science Programme, Centre of Diagnostic, Therapeutic and Investigative Studies, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia.
Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Malaysia.
Toxics. 2021 Jul 5;9(7):159. doi: 10.3390/toxics9070159.
The adverse effects of maternal pesticides exposure on the progeny is very well established. However, the impact of paternal exposure to pesticides such as Fenitrothion (FNT) on the histomorphometry of progeny's organs in unexposed mothers are much less well studied. Therefore, this study aims to evaluate the effects of paternal FNT exposure on the sperm quality of the parent rat and its effects on the histomorphometry of the progeny's organs. Randomly, male Sprague Dawley rats ( = 24) categorized as F0 were distributed equally into three groups namely Control, FNT-10, and FNT-20. Control received 1 mL/kg corn oil while FNT-10 and FNT-20 received 10 mg/kg and 20 mg/kg of FNT, respectively, via oral force feeding for 28 consecutive days. At the end of the study, male rats were mated with unexposed female rats and the male rats were sacrificed to obtain sperm for sperm characterization and DNA damage evaluation. Meanwhile, the rats' progeny (F1) namely Control, FNT-10, and FNT-20 were left to grow until postnatal day 70 before being sacrificed to obtain the matured organs for histology and morphometric analysis. Our results showed that both doses of FNT reduced sperm quality and caused DNA fragmentation in F0 rats compared with the control group ( < 0.05). The number of Leydig cells as well as the diameter of the seminiferous tubules and glomerulus of the FNT-20 group had significantly decreased ( < 0.05) compared with the Control group. The Bowman's space of the FNT-20 group had significantly increased ( < 0.05) compared with the FNT-10 and Control groups. Therefore, paternal exposure to FNT reduced the sperm quality and increased sperm DNA fragmentation in F0 male Sprague Dawley rats and altered the histology and morphometry of the selected organs in the F1 progeny.
母体接触农药对后代的不良影响已得到充分证实。然而，父亲接触农药如杀螟硫磷（FNT）对未接触农药的母亲所产后代器官组织形态计量学的影响则研究得少得多。因此，本研究旨在评估父亲接触FNT对亲代大鼠精子质量的影响及其对后代器官组织形态计量学的影响。将24只雄性斯普拉格-道利大鼠随机分为F0组，平均分为三组，即对照组、FNT - 10组和FNT - 20组。对照组给予1 mL/kg玉米油，而FNT - 10组和FNT - 20组分别通过口服灌胃连续28天给予10 mg/kg和20 mg/kg的FNT。在研究结束时，将雄性大鼠与未接触农药的雌性大鼠交配，然后处死雄性大鼠以获取精子进行精子特征分析和DNA损伤评估。同时，将大鼠后代（F1）即对照组、FNT - 10组和FNT - 20组饲养至出生后第70天，然后处死以获取成熟器官进行组织学和形态计量学分析。我们的结果表明，与对照组相比，两种剂量的FNT均降低了F0大鼠的精子质量并导致DNA片段化（P < 0.05）。与对照组相比，FNT - 20组的睾丸间质细胞数量以及曲细精管和肾小球直径显著降低（P < 0.05）。与FNT - 10组和对照组相比，FNT - 20组的鲍曼间隙显著增加（P < 0.05）。因此，父亲接触FNT会降低F0雄性斯普拉格-道利大鼠的精子质量并增加精子DNA片段化，并改变F1后代所选器官的组织学和形态计量学。
