Faculty of Medicine, Department of Clinical Pathololgy, Alexandria University, Alexandria, Egypt.
Andrologia. 2012 May;44 Suppl 1:484-9. doi: 10.1111/j.1439-0272.2011.01212.x. Epub 2011 Aug 1.
The aim of the present study was to evaluate the association of Glycine102Serine molecular variant of luteinising hormone (LH) β-subunit gene with infertility in Egyptian males and to assess the impact of oxidative stress in association with Glycine102Serine molecular variant on male infertility. Fifty men with idiopathic oligozoospermia were enrolled in the study. The control group consisted of 39 men with normal semen parameters and proven fertility. Variable oxidative status markers were evaluated. Glycine102Serine variant was investigated using restriction fragment length polymorphism-polymerase chain reaction. The study concluded that Glycine102Serine variant of LH β-subunit gene is relatively common among infertile Egyptian males and might be implicated in some Egyptian infertility cases. In addition, the association of this variant, in some cases, with disturbed oxidative status (decreased seminal plasma antioxidants' level and increased sperm lipid peroxidation) probably have a significant role in accentuation of infertility in those particular cases.
本研究旨在评估黄体生成素（LH）β亚单位基因甘氨酸 102 丝氨酸分子变异与埃及男性不育之间的关系，并评估氧化应激与甘氨酸 102 丝氨酸分子变异对男性不育的影响。50 名特发性少精子症患者纳入研究。对照组由 39 名具有正常精液参数和生育能力的男性组成。评估了可变氧化状态标志物。使用限制性片段长度多态性聚合酶链反应研究甘氨酸 102 丝氨酸变异。研究表明，LHβ亚单位基因甘氨酸 102 丝氨酸变异在埃及不育男性中相对常见，可能与一些埃及不育病例有关。此外，在某些情况下，这种变异与氧化应激紊乱（精液抗氧化剂水平降低和精子脂质过氧化增加）相关，可能在这些特定病例中加重不育症方面具有重要作用。
