Davar Robab, Tabibnejad Nasim, Kalantar Seyed Mehdi, Sheikhha Mohammad Hasan
Research and Clinical Center for Infertility, Shahid Sdoughi University of Medical Sciences, Yazd, Iran.
Iran J Reprod Med. 2014 Oct;12(10):667-72.
Despite extensive progress in IVF techniques, one of the most difficult problems is the variability in the response to controlled ovarian hyperstimulation (COH). Recent studies show the effects of individual genetic variability on COH outcome.
To evaluate the correlation between LHβ G1502A polymorphisms in exon 3 of the LH gene and ovarian response to COH.
A total of 220 women treated with a long protocol for ovarian stimulation were studied. Three genotypes of GG, GA and AA were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.
In total, 34 (17%) patients were poor responders, 154 (77%) were normal responders and 12 (6%) were hyper responders. The most frequent genotype was GA (55.5%) whereas 44.5% of patients showed GG genotype and there was no patient with AA genotype. In total 54.5% of normal responders, 61.8% of poor responders and 50% of hyper responders showed GA genotype.
Our results did not establish a significant relationship between this polymorphism and the ovarian response. Therefore it is still very difficult to use the genotype of patients for prediction of the ovarian response to stimulation.
尽管体外受精（IVF）技术取得了长足进展，但最困难的问题之一是对控制性卵巢过度刺激（COH）的反应存在变异性。最近的研究表明个体基因变异性对COH结果的影响。
评估促黄体生成素（LH）基因第3外显子中LHβ G1502A多态性与卵巢对COH反应之间的相关性。
共研究了220例接受长方案卵巢刺激治疗的女性。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析检测了GG、GA和AA三种基因型。
总共34例（17%）患者为低反应者，154例（77%）为正常反应者，12例（6%）为高反应者。最常见的基因型是GA（55.5%），而44.5%的患者表现为GG基因型，没有患者为AA基因型。正常反应者中54.5%、低反应者中61.8%、高反应者中50%表现为GA基因型。
我们的结果未证实这种多态性与卵巢反应之间存在显著关系。因此，利用患者基因型预测卵巢对刺激的反应仍然非常困难。
