活非侵袭性志贺氏痢疾杆菌 1 株在豚鼠结肠炎模型中诱导同源保护免疫。
Live non-invasive Shigella dysenteriae 1 strain induces homologous protective immunity in a guinea pig colitis model.
机构信息
National Institute of Cholera and Enteric Diseases, Kolkata, India.
出版信息
Microbiol Immunol. 2011 Oct;55(10):683-93. doi: 10.1111/j.1348-0421.2011.00371.x.
A non-invasive live transconjugant Shigella hybrid (LTSHΔstx) strain was constructed from a Shiga toxin gene deleted mutant of Shigella dysenteriae 1 by introducing a plasmid vector pPR1347 that carried a lipopolysaccharide biosynthesis gene (rfb and rfc) of Salmonella typhimurium. In guinea pigs, four successive oral administrations of LTSH Δstx showed complete protection against rectal challenge with wild type S. dysenteriae 1 strain. Exponential increase of the serum IgG and IgA titer against lipopolysaccharide of LTSH Δstx was observed during immunization, peaked on day 28 and remained at that level until day 35 after the initiation of the immunization. In intestinal lavage of the immunized animals, significant increase of IgA titer against lipopolysaccharide of LTSH Δstx was also observed. These data suggested that LTSH Δstx could be a useful candidate to induce protective immunity against S. dysenteriae 1 infection.
构建了一种非侵入性活体穿梭接合子志贺氏菌杂种（LTSHΔstx）菌株，该菌株是通过引入携带鼠伤寒沙门氏菌脂多糖生物合成基因（rfb 和 rfc）的质粒载体 pPR1347，从志贺氏痢疾杆菌的志贺毒素基因缺失突变株中获得的。在豚鼠中，连续 4 次口服给予 LTSHΔstx 可完全抵抗野生型志贺氏痢疾杆菌 1 株的直肠攻击。在免疫过程中观察到针对 LPS 的血清 IgG 和 IgA 滴度的指数增加，在免疫开始后第 28 天达到峰值，并持续至第 35 天。在免疫动物的肠灌洗液中，也观察到针对 LPS 的 IgA 滴度的显著增加。这些数据表明，LTSHΔstx 可能是诱导针对志贺氏痢疾杆菌 1 感染的保护性免疫的一种有用候选物。
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