National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme XM.
Microbiol Immunol. 2013 Nov;57(11):762-71. doi: 10.1111/1348-0421.12095.
The protective efficacy of and immune response to heat-killed cells of monovalent and hexavalent mixtures of six serogroups/serotypes of Shigella strains (Shigella dysenteriae 1, Shigella flexneri 2a, S. flexneri 3a, S. flexneri 6, Shigella boydii 4, and Shigella sonnei) were examined in a guinea pig colitis model. A monovalent or hexavalent mixture containing 1 × 10(7) of each serogroup/serotype of heat-killed Shigella cells was administered orally on Days 0, 7, 14 and 21. On Day 28, the immunized animals were challenged rectally with 1 × 10(9) live virulent cells of each of the six Shigella serogroups/serotypes. In all immunized groups, significant levels of protection were observed after these challenges. The serum titers of IgG and IgA against the lipopolysaccharide of each of the six Shigella serogroups/serotypes increased exponential during the course of immunization. High IgA titers against the lipopolysaccharide of each of the six Shigella serogroups/serotypes were also observed in intestinal lavage fluid from all immunized animals. These data indicate that a hexavalent mixture of heat-killed cells of the six Shigella serogroups/serotypes studied would be a possible broad-spectrum candidate vaccine against shigellosis.
在豚鼠结肠炎模型中，研究了单价和六价混合的 6 种血清型/血清型志贺氏菌菌株（志贺氏痢疾杆菌 1、志贺氏福氏 2a、福氏 3a、福氏 6、鲍氏志贺氏菌 4 和宋内志贺氏菌）的热灭活细胞的保护效力和免疫应答。单价或六价混合物在第 0、7、14 和 21 天口服给予 1×10(7)个每种血清型/血清型的热灭活志贺氏菌细胞。在第 28 天，用 1×10(9)个活的每种 6 种志贺氏菌血清型/血清型的毒力细胞对免疫动物进行直肠挑战。在所有免疫组中，在这些挑战后观察到显著水平的保护。在免疫过程中，针对 6 种志贺氏菌血清型/血清型的脂多糖的 IgG 和 IgA 血清滴度呈指数增加。在所有免疫动物的肠灌洗液中也观察到针对 6 种志贺氏菌血清型/血清型的脂多糖的高 IgA 滴度。这些数据表明，研究的 6 种志贺氏菌血清型/血清型的热灭活细胞的六价混合物可能是一种针对志贺氏菌病的广谱候选疫苗。
