Chen Ai-zhen, Liu Nan, Huang Huan, Lin Fei-fei, Liu De-shan, Lin Xiao-hui
Department of Neurology, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Aug;27(8):868-71.
To investigate the effects of adipose-derived stem cell (ADSC) transplanting on the outgrowth of neuronal axons and the expressions of GFAP, Neuritin, NF-200 in the brain post focal cerebral ischemia in rats.
54 male adult Sprague-Dawley rats were randomly divided into 3 groups: sham-operated group, middle cerebral artery occlusion (MCAO) group and MCAO+ADSC-treated group (n=18 in each group). A permenant focal cerebal ischemia model was established using modified Longa's method ADSC was labeled by DAPI before the transplantation. One day after MCAO, 30 μL of cell suspension containing 1×10(6); cells were injected into the lateral ventricle of MCAO+ADSC-treated group. At 7 d, 14 d and 28 d after MCAO, the expressions of GFAP, Neuritin and NF-200 were detected in ischemic region by Western blot and Immunofluorescence analysis.
DAPI staining positive cells were observed around the cerebral infarcted area in the ADSC group. The expressions of Neuritin, NF200 were higher, but GFAP was lower than that of the MCAO group at 7 d, 14 d and 28 d (P<0.05).
The transplantation of ADSC can induce regeneration and repairment of impaired neuronal axons in rat brain after cerebral ischemia, partly by inhibiting the expression of GFAP and enhancing the expressions of Neuritin, NF-200 in the brain.
探讨脂肪干细胞（ADSC）移植对大鼠局灶性脑缺血后神经轴突生长及脑内胶质纤维酸性蛋白（GFAP）、神经生长素（Neuritin）、神经丝蛋白200（NF-200）表达的影响。
54只成年雄性Sprague-Dawley大鼠随机分为3组：假手术组、大脑中动脉闭塞（MCAO）组和MCAO+ADSC治疗组（每组18只）。采用改良的Longa法建立永久性局灶性脑缺血模型，ADSC在移植前用DAPI标记。MCAO术后1天，向MCAO+ADSC治疗组侧脑室内注射含1×10⁶个细胞的30 μL细胞悬液。MCAO术后7天、14天和28天，通过蛋白质免疫印迹法和免疫荧光分析检测缺血区域GFAP、Neuritin和NF-200的表达。
ADSC组在脑梗死灶周围观察到DAPI染色阳性细胞。在术后7天、14天和28天，Neuritin、NF200的表达高于MCAO组，而GFAP的表达低于MCAO组（P<0.05）。
ADSC移植可诱导大鼠脑缺血后受损神经轴突的再生和修复，其机制可能部分是通过抑制脑内GFAP的表达，增强Neuritin、NF-200的表达来实现的。
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