Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, Brazil.
Talanta. 2011 Sep 15;85(3):1364-8. doi: 10.1016/j.talanta.2011.06.013. Epub 2011 Jun 15.
An analytical procedure for multiple standard additions of arsenic species using sequential injection analysis (SIA) is proposed for their quantification in seafood extracts. SIA presented flexibility for generating multiple specie standards at the ng mL(-1) concentration level by adding different volumes of As(III), As(V), monomethylarsonic (MMA) and dimethylarsinic (DMA) to the sample. The mixed sample plus standard solutions were delivered from SIA to fill the HPLC injection loop. Subsequently, As species were separated by HPLC and analyzed by atomic fluorescence spectrometry (AFS). The proposed system comprised two independently controlled modules, with the HPLC loop acting as the intermediary device. The analytical frequency was enhanced by combining the actions of both modules. While the added sample was flowing through the chromatographic column towards the detection system, the SIA program started performing the standard additions to another sample. The proposed method was applied to spoiled seafood extracts. Detection limits based on 3σ for As(III), As(V), MMA and DMA were 0.023, 0.39, 0.45 and 1.0 ng mL(-1), respectively.
提出了一种利用顺序注射分析（SIA）进行多种砷形态标准加入的分析程序，以定量测定海鲜提取物中的砷形态。SIA 通过向样品中加入不同体积的 As(III)、As(V)、一甲基砷酸（MMA）和二甲基砷酸（DMA），在 ng mL(-1)浓度水平上灵活地生成多种标准品。混合样品和标准溶液由 SIA 输送，以填充 HPLC 进样环。随后，通过 HPLC 分离砷形态，并通过原子荧光光谱法（AFS）进行分析。所提出的系统由两个独立控制的模块组成，HPLC 环作为中间设备。通过组合两个模块的操作，可以提高分析频率。当加入的样品流经色谱柱进入检测系统时，SIA 程序开始对另一个样品进行标准加入。该方法应用于变质海鲜提取物。基于 3σ 的 As(III)、As(V)、MMA 和 DMA 的检测限分别为 0.023、0.39、0.45 和 1.0 ng mL(-1)。
