Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, China.
Talanta. 2011 Sep 15;85(3):1672-9. doi: 10.1016/j.talanta.2011.06.067. Epub 2011 Jul 2.
A novel electrochemical sensor based on molecularly imprinted polymer film has been developed for aspirin detection. The sensitive film was prepared by co-polymerization of p-aminothiophenol (p-ATP) and HAuCl(4) on the Au electrode surface. First, p-ATP was self-assembled on the Au electrode surface by the formation of Au-S bonds. Then, the acetylsalicylic acid (ASA) template was assembled onto the monolayer of p-ATP through the hydrogen-bonding interaction between amino group (p-ATP) and oxygen (ASA). Finally, a conductive hybrid membrane was fabricated at the surface of Au electrode by the co-polymerization in the mixing solution containing additional p-ATP, HAuCl(4) and ASA template. Meanwhile, the ASA was spontaneously imprinted into the poly-aminothiophenol gold nanoparticles (PATP-AuNPs) complex film. The amount of imprinted sites at the PATP-AuNPs film significantly increases due to the additional replenishment of ASA templates. With the significant increasing of imprinted sites and doped gold nanoparticles, the sensitivity of the molecular imprinted polymer (MIP) electrode gradually increased. The molecularly imprinted sensor was characterized by electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), and cyclic voltammetry (CV). The linear relationships between current and logarithmic concentration were obtained in the range from 1 nmol L(-1) to 0.1 μmol L(-1) and 0.7 μmol L(-1) to 0.1 mmol L(-1). The detection limit of 0.3 nmol L(-1) was achieved. This molecularly imprinted sensor for the determination of ASA has high sensitivity, good selectivity and reproducibility, with the testing in some biological fluids also has good selectivity and recovery.
一种基于分子印迹聚合物膜的新型电化学传感器已被开发用于阿司匹林检测。敏感膜是通过在 Au 电极表面共聚 p-氨基苯硫酚（p-ATP）和 HAuCl4 制备的。首先，p-ATP 通过 Au-S 键形成自组装在 Au 电极表面上。然后，乙酰水杨酸（ASA）模板通过氨基（p-ATP）和氧（ASA）之间的氢键相互作用组装到 p-ATP 的单层上。最后，通过在含有额外 p-ATP、HAuCl4 和 ASA 模板的混合溶液中进行共聚，在 Au 电极表面上制备了导电混合膜。同时，ASA 自发地印迹到聚氨基苯硫酚金纳米粒子（PATP-AuNPs）复合膜中。由于额外补充 ASA 模板，印迹位点的数量在 PATP-AuNPs 膜中显著增加。随着印迹位点和掺杂金纳米粒子数量的显著增加，分子印迹聚合物（MIP）电极的灵敏度逐渐增加。通过电化学阻抗谱（EIS）、差分脉冲伏安法（DPV）和循环伏安法（CV）对分子印迹传感器进行了表征。在 1 nmol L-1 至 0.1 μmol L-1 和 0.7 μmol L-1 至 0.1 mmol L-1 的范围内，获得了电流与对数浓度之间的线性关系。检测限达到 0.3 nmol L-1。该用于测定 ASA 的分子印迹传感器具有高灵敏度、良好的选择性和重现性，在一些生物流体中的测试也具有良好的选择性和回收率。
