Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China.
Biosens Bioelectron. 2012 Oct-Dec;38(1):163-9. doi: 10.1016/j.bios.2012.05.017. Epub 2012 May 24.
A molecularly imprinted electrochemical sensor was fabricated based on gold electrode decorated by chitosan-platinum nanoparticles (CS-PtNPs) and graphene-gold nanoparticles (GR-AuNPs) nanocomposites for convenient and sensitive determination of erythromycin. The synergistic effects of CS-PtNPs and GR-AuNPs nanocomposites improved the electrochemical response and the sensitivity of the sensor. The molecularly imprinted polymers (MIPs) were prepared by HAuCl(4), 2-mercaptonicotinic acid (MNA) and erythromycin. Erythromycin and MNA were used as template molecule and functional monomer, respectively. They were first assembled on the surface of GR-AuNPs/CS-PtNPs/gold electrode by the formation of Au-S bonds and hydrogen-bonding interactions. Then the MIPs were formed by electropolymerization of HAuCl(4), MNA and erythromycin. The sensor was characterized by cyclic voltammetry (CV), scanning electron microscope (SEM), UV-visible (UV-vis) absorption speactra and amperometry. The linear range of the sensor was from 7.0 × 10(-8)mol/L-9.0 × 10(-5)mol/L, with the limit of detection (LOD) of 2.3 × 10(-8)mol/L (S/N=3). The sensor showed high selectivity, excellent stability and good reproducibility for the determination of erythromycin, and it was successfully applied to the detection of erythromycin in real spiked samples.
基于壳聚糖-铂纳米粒子（CS-PtNPs）和石墨烯-金纳米粒子（GR-AuNPs）纳米复合材料修饰的金电极制备了一种分子印迹电化学传感器，用于方便、灵敏地测定红霉素。CS-PtNPs 和 GR-AuNPs 纳米复合材料的协同作用提高了传感器的电化学响应和灵敏度。印迹聚合物（MIPs）是通过 HAuCl(4)、2-巯基烟酸（MNA）和红霉素制备的。红霉素和 MNA 分别用作模板分子和功能单体。它们首先通过 Au-S 键和氢键相互作用组装在 GR-AuNPs/CS-PtNPs/金电极表面。然后，通过 HAuCl(4)、MNA 和红霉素的电聚合形成 MIPs。通过循环伏安法（CV）、扫描电子显微镜（SEM）、紫外-可见（UV-vis）吸收光谱和安培法对传感器进行了表征。传感器的线性范围为 7.0×10(-8)mol/L-9.0×10(-5)mol/L，检测限（LOD）为 2.3×10(-8)mol/L（S/N=3）。该传感器对红霉素的测定具有高选择性、优异的稳定性和良好的重现性，成功应用于实际加标样品中红霉素的检测。
