AgResearch, Hopkirk Research Institute, Private Bag 11008, Palmerston North 4442, New Zealand.
Tuberculosis (Edinb). 2011 Sep;91(5):400-5. doi: 10.1016/j.tube.2011.07.001. Epub 2011 Jul 31.
Studies were undertaken to determine whether a dose of oral Mycobacterium bovis bacillus Calmette-Guérin (BCG) which did not induce skin test reactivity could protect cattle against bovine tuberculosis (TB). Groups of calves (n = 9) were vaccinated by administering 10(8), 10(7) or 10(6) colony forming units (CFU) of BCG orally or 10(6) CFU subcutaneous (s.c.) BCG. A control group (n = 10) was not vaccinated. All animals were challenged with M. bovis 18 weeks after vaccination and euthanized and necropsied at 16 weeks following challenge. Positive responses in the single cervical tuberculin skin test (severe interpretation) at 15 weeks post-vaccination were only observed in the s.c. BCG and 10(8) CFU oral BCG groups (four of nine animals/group). Following experimental challenge with M. bovis, both these BCG-vaccinated groups had significant reductions in lesion scores and bacterial counts whereas there was no protection in calves vaccinated with oral doses of 10(6) or 10(7) CFU of BCG. In conclusion, low oral doses of BCG did not induce skin test responses, IFN-γ responses or protection against TB, however, in the BCG vaccine groups where protection was observed, there was no correlation between protection and skin test responses or IFN-γ responses.
研究旨在确定口服牛分枝杆菌卡介苗（BCG）剂量是否可以保护牛免受牛结核病（TB）的侵害，该剂量不会引起皮肤试验反应。将小牛（n=9）分为 4 组，通过口服给予 10（8）、10（7）或 10（6）个菌落形成单位（CFU）的 BCG 或皮下（s.c.）接种 10（6）个 CFU 的 BCG 进行免疫接种。对照组（n=10）未接种。所有动物在接种后 18 周用 M. bovis 进行攻毒，并在攻毒后 16 周安乐死和剖检。在接种后 15 周的单次颈结核菌素皮肤试验（严重解释）中仅观察到皮下 BCG 和 10（8）CFU 口服 BCG 组（每组 9 只动物中的 4 只）出现阳性反应。用 M. bovis 进行实验性攻毒后，这两个 BCG 疫苗接种组的病变评分和细菌计数均显著降低，而口服给予 10（6）或 10（7）CFU 的 BCG 疫苗接种的小牛没有得到保护。总之，低剂量口服 BCG 既不能诱导皮肤试验反应，也不能诱导 IFN-γ 反应或预防 TB，但在观察到保护的 BCG 疫苗接种组中，保护与皮肤试验反应或 IFN-γ 反应之间没有相关性。
