Wedlock D Neil, Denis Michel, Vordermeier H Martin, Hewinson R Glyn, Buddle Bryce M
AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):50-8. doi: 10.1016/j.vetimm.2007.04.005. Epub 2007 Apr 19.
A number of studies have demonstrated significant protection of cattle against bovine tuberculosis following vaccination with the Pasteur strain of Mycobacterium bovis bacille Calmete-Guerin (BCG). However, it is unclear if other daughter strains of BCG are as effective, which is an important issue to resolve for a variety of regulatory compliance issues. This study compared the protective immune responses to bovine tuberculosis induced in cattle vaccinated with BCG Danish with those induced by BCG Pasteur. Groups of calves (n=10) were vaccinated with 10(6) colony forming units (CFU) BCG Pasteur prepared from a fresh liquid culture, 10(6) CFU BCG Danish prepared from a fresh liquid culture or 0.4 mg of reconstituted freeze-dried culture of BCG Danish. Another group (n=10) served as non-vaccinated controls. BCG Pasteur induced significantly higher and more sustained levels of bovine purified protein derivative (PPD)-specific gamma interferon (IFN-gamma) in whole-blood cultures following vaccination compared to either fresh culture BCG Danish or freeze-dried BCG Danish. Vaccination with a fresh culture of BCG Pasteur, fresh culture BCG Danish and freeze-dried BCG Danish gave a significant enhancement in three, four and three pathological and microbiological parameters of protection, respectively, compared to the non-vaccinated group. These results demonstrate the Danish strain of BCG is a viable alternative to BCG Pasteur for vaccination of cattle as both strains had similar efficacy and there was little difference between freshly cultured and freeze-dried formulation of BCG Danish. The results also show that post-vaccination antigen-specific IFN-gamma levels in whole blood is not always a reliable indicator of protection against a subsequent virulent challenge.
多项研究表明，用牛型结核分枝杆菌卡介苗（BCG）巴斯德菌株对牛进行疫苗接种后，可对牛结核病产生显著的保护作用。然而，尚不清楚卡介苗的其他子代菌株是否同样有效，这是解决各种监管合规问题的一个重要待解决事项。本研究比较了用丹麦卡介苗接种的牛与用巴斯德卡介苗接种的牛对牛结核病诱导的保护性免疫反应。将犊牛分组（每组n = 10），分别用从新鲜液体培养物制备的10⁶菌落形成单位（CFU）巴斯德卡介苗、从新鲜液体培养物制备的10⁶CFU丹麦卡介苗或0.4mg重组冻干丹麦卡介苗培养物进行接种。另一组（n = 10）作为未接种疫苗的对照。与新鲜培养的丹麦卡介苗或冻干丹麦卡介苗相比，接种后，巴斯德卡介苗在全血培养物中诱导的牛纯化蛋白衍生物（PPD）特异性γ干扰素（IFN-γ）水平显著更高且更持久。与未接种疫苗的组相比，用新鲜培养的巴斯德卡介苗、新鲜培养的丹麦卡介苗和冻干丹麦卡介苗接种分别在三个、四个和三个病理及微生物学保护参数方面有显著增强。这些结果表明，丹麦卡介苗菌株是牛接种疫苗时巴斯德卡介苗的可行替代物，因为两种菌株具有相似的效力，并且新鲜培养和冻干的丹麦卡介苗制剂之间几乎没有差异。结果还表明，接种疫苗后全血中抗原特异性IFN-γ水平并不总是针对后续强毒攻击的保护的可靠指标。
