Buddle Bryce M, Aldwell Frank E, Skinner Margot A, de Lisle Geoffrey W, Denis Michel, Vordermeier H Martin, Hewinson R Glyn, Wedlock D Neil
AgResearch, Wallaceville Animal Research Centre, P.O. Box 40063, Upper Hutt, New Zealand.
Vaccine. 2005 May 20;23(27):3581-9. doi: 10.1016/j.vaccine.2005.01.150.
Cattle were given Mycobacterium bovis bacillus Calmette-Guerin (BCG) in a lipid-based formulation via the oral route and tested for immune responses and protection against a challenge with virulent M. bovis. Calves were vaccinated by orally administering a pellet containing 10(8) colony forming units (CFU) of BCG, or 10 pellets containing a total of 10(9) CFU of BCG, whereas positive controls were injected subcutaneously with 10(6) CFU of BCG. All of the subcutaneously vaccinated calves produced positive responses in the caudal fold tuberculin skin test at 8 weeks after vaccination, whereas only 3/9 of the low dose and 6/10 of the high dose orally-vaccinated animals produced positive reactions. None of the animals produced positive reactions to the mycobacterial antigens, ESAT-6 and CFP10 in the interferon-gamma (IFN-gamma) test and only a total of four of the BCG-vaccinated animals produced positive responses in either the standard IFN-gamma or comparative cervical skin test. Oral administration of 10 pellets of lipid-formulated BCG to cattle induced a significant level of protection against bovine tuberculosis compared to that observed in non-vaccinated animals and this level was similar to that seen in the BCG subcutaneously vaccinated animals. Oral vaccination of BCG in a lipid-formulation to calves was shown to induce some positive tuberculin skin test reactions, but could also induce protection against bovine tuberculosis.
通过口服途径给牛接种基于脂质制剂的卡介苗（BCG），并测试其免疫反应以及对强毒牛分枝杆菌攻击的保护作用。给犊牛口服含10⁸菌落形成单位（CFU）卡介苗的药丸，或口服含总共10⁹CFU卡介苗的10粒药丸进行疫苗接种，而阳性对照则皮下注射10⁶CFU卡介苗。所有皮下接种疫苗的犊牛在接种疫苗后8周的尾褶结核菌素皮肤试验中均产生阳性反应，而低剂量口服疫苗的动物中只有3/9、高剂量口服疫苗的动物中只有6/10产生阳性反应。在干扰素-γ（IFN-γ）试验中，没有动物对结核分枝杆菌抗原ESAT-6和CFP10产生阳性反应，并且在卡介苗接种的动物中，总共只有四只在标准IFN-γ或比较性颈部皮肤试验中产生阳性反应。与未接种疫苗的动物相比，给牛口服10粒脂质制剂卡介苗可诱导出显著水平的抗牛结核病保护作用，且该水平与皮下接种卡介苗的动物相似。给犊牛口服脂质制剂卡介苗显示可诱导一些阳性结核菌素皮肤试验反应，但也可诱导出抗牛结核病的保护作用。
