Municipal Key Laboratory of Microorganism and Environmental Engineering, Ningbo, Zhejiang 315100, China.
Evid Based Complement Alternat Med. 2011;2011:209406. doi: 10.1155/2011/209406. Epub 2011 Jul 17.
For the first time, a mollicute-like organism (MLO) was cultured from moribund mud crabs (Scylla serrata) during an outbreak of clearwater disease in Zhejiang Province, China. The MLO displayed a fried-egg colony morphology in culture, did not possess a cell wall, and was not retained by 0.45 μm and 0.2 μm filters. It was able to ferment glucose, sucrose, lactose, and maltose, but it did not utilize arginine and urea. The MLO grew in the absence of bovine serum and was not susceptible to digitonin. Sequence analysis of the 16S rRNA gene revealed that this MLO had 99% identity with Acholeplasma laidlawii PG-8A, which indicates that the organism isolated from mud crabs is a member of the genus Acholeplasma.
首次从浙江省暴发的清水病濒死泥蟹（锯缘青蟹）中培养出一种类似柔膜菌的生物体（MLO）。该 MLO 在培养中呈现出煎蛋状菌落形态，没有细胞壁，不能被 0.45μm 和 0.2μm 过滤器截留。它能够发酵葡萄糖、蔗糖、乳糖和麦芽糖，但不能利用精氨酸和尿素。该 MLO 在没有牛血清的情况下生长，并且不易受胆酸钠影响。16S rRNA 基因序列分析表明，该 MLO 与 Acholeplasma laidlawii PG-8A 的同源性为 99%，这表明从泥蟹中分离出的生物体是 Acholeplasma 属的一员。
