Balasubramanian C P, Cubelio S S, Mohanlal D L, Ponniah A G, Kumar Raj, Bineesh K K, Ravichandran P, Gopalakrishnan A, Mandal A, Jena J K
a Central Institute of Brackishwater Aquaculture , Chennai , Tamil Nadu , India .
b National Bureau of Fish Genetic Resources Cochin Unit , Kochi , Kerala , India , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):270-5. doi: 10.3109/19401736.2014.892076. Epub 2014 Mar 24.
For several years, mud crabs of genus Scylla have been misidentified owing to their high morphological plasticity and the absence of distinct morphological diagnostic characters. The taxonomic confusion of genus Scylla de Haan is considered to be a primary constraint to the development of aquaculture. Although genus Scylla was revised using morphological and genetic characteristics, taxonomy of Scylla species occurring in India is still not clear. In this study, partial sequences of two mitochondrial genes, 16S rRNA and CO1 (Cytochrome C oxidase subunit I) in populations of Scylla spp. obtained from eleven locations along the Indian coast were used to differentiate and resolve taxonomical ambiguity of the mud crab species in India. The sequences were compared with previously published sequences of Scylla spp. Both trees generated based on 16S rRNA and CO1 indicated that all S. tranquebarica morphotypes obtained during this study and S. tranquebarica sequences submitted previously from Indian waters reciprocally monophyletic with reference sequence of S. serrata. Both sequence data and morphological characters revealed that the species S. serrata (Forskal) is the most abundant followed by S. olivacea. Further, the 16S rRNA and COI haplotypes of Indian S. tranquebarica obtained in the study significantly differed with the known S. tranquebarica by 6.7% and 10.6% respectively whereas it differed with known S. serrata by 0.0-0.7% only, a difference that was not statistically significant. From these studies it is clear that "S. tranquebarica" commonly reported from India should be S. serrata (Forskal).
多年来，锯缘青蟹属的泥蟹因其高度的形态可塑性以及缺乏明显的形态诊断特征而被误认。锯缘青蟹属的分类混乱被认为是水产养殖发展的主要制约因素。尽管已利用形态学和遗传学特征对锯缘青蟹属进行了修订，但印度锯缘青蟹种类的分类仍不明确。在本研究中，从印度沿海11个地点采集的锯缘青蟹种群的两个线粒体基因16S rRNA和CO1（细胞色素C氧化酶亚基I）的部分序列，被用于区分和解决印度泥蟹种类的分类歧义。将这些序列与先前发表的锯缘青蟹属序列进行了比较。基于16S rRNA和CO1生成的两棵树均表明，本研究期间获得的所有拟穴青蟹形态型以及先前从印度水域提交的拟穴青蟹序列与锯缘青蟹的参考序列相互单系。序列数据和形态特征均显示，锯缘青蟹（Forskal）是最丰富的物种，其次是榄绿青蟹。此外，本研究中获得的印度拟穴青蟹的16S rRNA和COI单倍型与已知的拟穴青蟹分别有6.7%和10.6%的显著差异，而与已知的锯缘青蟹仅相差0.0 - 0.7%，这种差异无统计学意义。从这些研究中可以清楚地看出，印度常见报道的“拟穴青蟹”应为锯缘青蟹（Forskal）。
