Gomes Otoni Moreira, Brasileiro Filho Geraldo, Salles Rodrigo Lolli Almeida, Linhares Rafaela Ervilha, Nazar Maria Cristina, Porto Luiz Alberto Bonjardim, Paixão Rafael de Mattos, Miranda Marcelo Heller
Department of Surgery, School of Medicine, UFMG, Brazil.
Acta Cir Bras. 2011 Aug;26(4):285-8. doi: 10.1590/s0102-86502011000400007.
To evaluate the histopathology alterations of the intestinal mucosa of rabbits submitted to mesenteric artery ischemia and reperfusion with and without ischemic preconditioning.
Two groups of ten male New Zealand white rabbits body (weight 2.2-3.0, average 2.5 kg). For mesenteric ischemia induction in all animals the small bowel and mesentery were cut 30cm and 60cm far from the gastroduodenal pyloric transition before the proximal mesenteric artery occlusion. In the Group 1 animals, the proximal mesenteric artery was occluded for 45 min with an atraumatic vascular clamp, followed by reperfusion for 30 min. In the Group 2 the 45 min ischemic phase was preceded by three cycles of ischemia (2 minutes each) alternated with three cycles of reperfusion (2 minutes each). For istopathology study small bowel biopsies were obtained before ischemia (control), after 45 min of mesenteric ischemia and at 30 min. of mesenteric artery reperfusion.
In the Group I animals, the followings histopathology grade results were observed: t1, mean 2,8; t2, mean 3,3. Using the Kruskal-Wallis non-parameter test, differences between t0 and t1 and t0 and t2 were significants (p<0.05), but not significant between t1 and t2 (p>0.05). In the Group 2 animals histopathology grade results were: t1 mean 2,6 and t2, mean 2,1. Differences between t0 and t1, t0 and t2 were significant (p<0.05). It was not observed differences (p>0.05) between results of t1 in both groups but histopathology injury observed in Group 1 t2 biopsies were higher (p<0.05) than observed in the same period (t2) of Group 2 animals.
Microscopic examination of the biopsies revealed significant evidence of preconditioning protection against small bowel wall ischemia-reperfusion injury.
评估接受肠系膜动脉缺血再灌注且有或无缺血预处理的家兔肠黏膜的组织病理学改变。
两组，每组十只雄性新西兰白兔（体重2.2 - 3.0千克，平均2.5千克）。为诱导所有动物发生肠系膜缺血，在肠系膜上动脉近端闭塞前，将小肠和肠系膜分别在距胃十二指肠幽门交界处30厘米和60厘米处切断。在第1组动物中，用无损伤血管夹将肠系膜上动脉闭塞45分钟，随后再灌注30分钟。在第2组中，45分钟的缺血期之前有三个缺血周期（每个周期2分钟），并与三个再灌注周期（每个周期2分钟）交替。为进行组织病理学研究，在缺血前（对照）、肠系膜缺血45分钟后以及肠系膜动脉再灌注30分钟时获取小肠活检组织。
在第1组动物中，观察到以下组织病理学分级结果：t1，平均2.8；t2，平均3.3。使用Kruskal - Wallis非参数检验，t0与t1以及t0与t2之间的差异具有显著性（p<0.05），但t1与t2之间差异不显著（p>0.05）。在第2组动物中，组织病理学分级结果为：t1平均2.6，t2平均2.1。t0与t1、t0与t2之间的差异具有显著性（p<0.05）。未观察到两组t1结果之间的差异（p>0.05），但第1组t2活检组织中观察到的组织病理学损伤高于第2组动物同期（t2）观察到的损伤（p<0.05）。
活检组织的显微镜检查显示有显著证据表明预处理可保护小肠壁免受缺血再灌注损伤。
