Dos Santos Carlos Henrique Marques, Pontes José Carlos Dorsa Vieira, Gomes Otoni Moreira, Miiji Luciana Nakao Odashiro, Bispo Marco Aurélio Feltrin
Federal University of Mato Grossodo Sul, Campo Grande, MS, Brazil.
Rev Bras Cir Cardiovasc. 2009 Apr-Jun;24(2):150-6. doi: 10.1590/s0102-76382009000200010.
To assess the preconditioning and postconditioning effect on intestinal mucosal lesions in rats undergone mesenteric ischemia and reperfusion procedure.
Thirty Wistar rats were studied and divided into three groups: Group A, 10 rats undergone mesenteric ischemia (30 minutes) and reperfusion (60 minutes); Group B, 10 rats undergone mesenteric ischemia and reperfusion preceded by ischemic preconditioning for three cycles of ischemia and reperfusion for two minutes each; Group C, 10 rats undergone mesenteric ischemia and reperfusion and, preceding the beginning of reperfusion, ischemic postconditioning was performed for three cycles of reperfusion and ischemia for two minutes each. Then, a segment of small intestine was resected for histological analysis. We assessed the results by Chiu et al. score and the statistical analysis was performed.
According to Chiu et al. score, the means of lesion degree were: In the group A, 3.5; Group B, 1.2; Group C, 1. The difference between group A with the groups B and C was considered statistically significant (P < 0.05).
Ischemic pre- and postconditioning were capable of minimizing - in a similar intensity - the tissue injury on the intestinal mucosa of rats undergone mesenteric ischemia and reperfusion process.
评估预处理和后处理对经历肠系膜缺血再灌注手术的大鼠肠黏膜损伤的影响。
对30只Wistar大鼠进行研究并分为三组：A组，10只大鼠经历肠系膜缺血（30分钟）和再灌注（60分钟）；B组，10只大鼠在经历肠系膜缺血和再灌注之前进行缺血预处理，即进行三个周期的缺血再灌注，每个周期两分钟；C组，10只大鼠经历肠系膜缺血和再灌注，在再灌注开始前进行缺血后处理，即进行三个周期的再灌注和缺血，每个周期两分钟。然后，切除一段小肠进行组织学分析。我们采用Chiu等人的评分法评估结果并进行统计分析。
根据Chiu等人的评分法，损伤程度的均值为：A组3.5；B组1.2；C组1。A组与B组和C组之间的差异具有统计学意义（P < 0.05）。
缺血预处理和后处理能够以相似的强度将经历肠系膜缺血再灌注过程的大鼠肠黏膜组织损伤降至最低。
