College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Amino Acids. 2012 Jun;42(6):2417-28. doi: 10.1007/s00726-011-1047-3. Epub 2011 Aug 2.
Taurine (Tau) is reported to have a key role in the regulation of the innate immune response and thus reduce tissue damage induced by bacterial infection. In this study, the effects of Tau on a rat model of mastitis induced by Streptococcus uberis (S. uberis) and the changes of T regulatory cells (Tregs) were assessed. Starting on gestation day 14 and continuing until parturition, 100 mg/kg of taurine (group TS) or an equal volume of physiological saline (group CS) was administered daily, per os. Seventy-two hours after parturition, rats were infused with approximately 100 cfu of S. uberis into each of two mammary glands. The results showed that the resultant inflammation, evidenced by swelling, secretory epithelial cell degeneration, increased adipose tissue and neutrophil (PMN) infiltration were evident in mammary tissue following injection with S. uberis. Pre-treatment with Tau attenuated these morphologic changes, the expression of interleukin (IL)-2, interferon (INF)-γ mRNA, myeloperoxidase (MPO) activity and N-acetyl-β-D-glucosaminidase (NAGase) in mammary tissue. The percentages of Foxp3+CD25+CD4+/lymphocytes (Tregs) were dramatically increased after the S. uberis challenge. Significant differences (P<0.05) were observed at 24, and 72 h post S. uberis-injection (PI) in CS. Pre-treatment further increased the percentage of Tregs and a significant difference between CS and TS (P<0.05) was apparent at 24 h PI. Our data indicate that in rats, Tau can be used to regulate the immune response following infection by S. uberis and consequently prevent mammary tissue damage by increasing Tregs.
牛磺酸（Tau）据报道在调节先天免疫反应中具有关键作用，从而减轻细菌感染引起的组织损伤。在这项研究中，评估了 Tau 对金黄色葡萄球菌（S. uberis）诱导的乳腺炎大鼠模型的影响以及调节性 T 细胞（Tregs）的变化。从妊娠第 14 天开始并持续到分娩，每天经口给予 100mg/kg 的牛磺酸（TS 组）或等量的生理盐水（CS 组）。分娩后 72 小时，将大约 100cfu 的 S. uberis 注入每只乳腺的两个乳腺中。结果表明，金黄色葡萄球菌注射后，乳腺组织中的炎症明显，表现为肿胀、分泌上皮细胞退化、脂肪组织增加和中性粒细胞（PMN）浸润。Tau 预处理减轻了这些形态变化，降低了乳腺组织中白细胞介素（IL）-2、干扰素（INF）-γ mRNA、髓过氧化物酶（MPO）活性和 N-乙酰-β-D-氨基葡萄糖苷酶（NAGase）的表达。金黄色葡萄球菌攻击后，Foxp3+CD25+CD4+/淋巴细胞（Tregs）的百分比显着增加。在 CS 中，在金黄色葡萄球菌注射后 24 和 72 小时（PI）观察到显着差异（P<0.05）。预处理进一步增加了 Tregs 的百分比，CS 和 TS 之间的差异具有统计学意义（P<0.05），在 24 h PI 时明显。我们的数据表明，在大鼠中，Tau 可用于调节金黄色葡萄球菌感染后的免疫反应，从而通过增加 Tregs 来预防乳腺组织损伤。
