Dai Bin, Zhang Yuan-shu, Ma Zi-li, Zheng Liu-hai, Li Shuang-jie, Dou Xin-hong, Gong Jian-sen, Miao Jin-feng
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Animal Husbandry and Veterinary Bureau of Dongyang, Dongyang 322100, China; Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225125, China.
J Zhejiang Univ Sci B. 2015 Jun;16(6):456-64. doi: 10.1631/jzus.B1400256.
Experiments were conducted to study the effects of dietary taurine and housing density on oviduct function in laying hens. Green-shell laying hens were randomly assigned to a free range group and two caged groups, one with low-density and the other with high-density housing. Each group was further divided into control (C) and taurine treatment (T) groups. All hens were fed the same basic diet except that the T groups' diet was supplemented with 0.1% taurine. The experiment lasted 15 d. Survival rates, laying rates, daily feed consumption, and daily weight gain were recorded. Histological changes, inflammatory mediator levels, and oxidation and anti-oxidation levels were determined. The results show that dietary taurine supplementation and reduced housing density significantly attenuated pathophysiological changes in the oviduct. Nuclear factor-κB (NF-κB) DNA binding activity increased significantly in the high-density housing group compared with the two other housing groups and was reduced by taurine supplementation. Tumor necrosis factor-α (TNF-α) mRNA expression in the high-density and low-density C and T groups increased significantly. In the free range and low-density groups, dietary taurine significantly reduced the expression of TNF-α mRNA. Supplementation with taurine decreased interferon-γ (IFN-γ) mRNA expression significantly in the low-density groups. Interleukin 4 (IL-4) mRNA expression was significantly higher in caged hens. IL-10 mRNA expression was higher in the high-density C group than in the free range and low-density C groups. Supplementation with taurine decreased IL-10 mRNA expression significantly in the high-density group and increased superoxide dismutase (SOD) activity in the free range hens. We conclude that taurine has important protective effects against oviduct damage. Reducing housing density also results in less oxidative stress, less inflammatory cell infiltration, and lower levels of inflammatory mediators in the oviduct. Therefore, both dietary taurine and reduced housing density can ameliorate oviduct injury, enhance oviduct health, and promote egg production in laying hens.
进行了实验以研究日粮牛磺酸和饲养密度对蛋鸡输卵管功能的影响。绿壳蛋鸡被随机分为放养组和两个笼养组，一个是低密度笼养组，另一个是高密度笼养组。每组进一步分为对照组（C）和牛磺酸处理组（T）。所有母鸡都饲喂相同的基础日粮，不同的是T组日粮添加了0.1%的牛磺酸。实验持续15天。记录存活率、产蛋率、日采食量和日增重。测定组织学变化、炎症介质水平以及氧化和抗氧化水平。结果表明，日粮添加牛磺酸和降低饲养密度可显著减轻输卵管的病理生理变化。与其他两个饲养组相比，高密度笼养组的核因子κB（NF-κB）DNA结合活性显著增加，而添加牛磺酸可降低该活性。高密度和低密度C组及T组的肿瘤坏死因子-α（TNF-α）mRNA表达均显著增加。在放养组和低密度组中，日粮牛磺酸显著降低了TNF-α mRNA的表达。在低密度组中，添加牛磺酸显著降低了干扰素-γ（IFN-γ）mRNA的表达。笼养母鸡的白细胞介素4（IL-4）mRNA表达显著更高。高密度C组的IL-10 mRNA表达高于放养组和低密度C组。在高密度组中，添加牛磺酸显著降低了IL-而在放养母鸡中增加了超氧化物歧化酶（SOD）活性。我们得出结论，牛磺酸对输卵管损伤具有重要的保护作用。降低饲养密度还可减少输卵管中的氧化应激、炎症细胞浸润和炎症介质水平。因此，日粮添加牛磺酸和降低饲养密度均可改善输卵管损伤，增强输卵管健康，并促进蛋鸡产蛋。 10 mRNA的表达，
