Klionsky D J, Emr S D
Division of Biology, California Institute of Technology, Pasadena 91125.
J Biol Chem. 1990 Apr 5;265(10):5349-52.
A number of inherited lysosomal diseases are known to result from missorting of lysosomal proteins. Considerable attention has been directed toward an understanding of this sorting pathway, and it has become apparent that different mechanisms are used for the sorting of lysosomal membrane and soluble proteins. Protein sorting to the yeast vacuole/lysosome provides a simple model system to study this process. We have mapped the first sorting signal in a vacuolar membrane protein, repressible alkaline phosphatase, and have shown it to be both necessary and sufficient for vacuolar delivery of this enzyme. The sorting information is confined to the transmembrane and cytoplasmic tail region of this type II integral membrane protein. The location of this sorting signal provides an explanation for some of the differences observed between membrane and soluble vacuolar protein sorting.
已知一些遗传性溶酶体疾病是由溶酶体蛋白分选错误导致的。人们对这种分选途径的理解给予了相当多的关注，并且很明显，溶酶体膜蛋白和可溶性蛋白的分选使用了不同的机制。蛋白质分选到酵母液泡/溶酶体提供了一个研究这一过程的简单模型系统。我们已经在一种液泡膜蛋白——可阻遏碱性磷酸酶中定位了第一个分选信号，并表明它对于该酶向液泡的转运既是必要的也是充分的。分选信息局限于这种II型整合膜蛋白的跨膜和胞质尾部区域。这个分选信号的位置为膜蛋白和可溶性液泡蛋白分选之间观察到的一些差异提供了解释。
