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哺乳动物RNA聚合酶II特异性转录所涉及的因子。转录因子IIF的纯化及亚基组成。

Factors involved in specific transcription by mammalian RNA polymerase II. Purification and subunit composition of transcription factor IIF.

作者信息

Flores O, Ha I, Reinberg D

机构信息

Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635.

出版信息

J Biol Chem. 1990 Apr 5;265(10):5629-34.

PMID:2180931
Abstract

Transcription factor IIF (TFIIF), a protein factor required for transcription of class II genes, has been purified to near homogeneity. TFIIF is a heterodimer of 30- and 78-kDa polypeptides. Both the 30- and 78-kDa polypeptides were necessary to reconstitute TFIIF activity. TFIIF activity eluted from a gel filtration column with an apparent molecular mass of 220 kDa, suggesting that the native factor exists as a dimer of the heterodimer.

摘要

转录因子IIF（TFIIF）是II类基因转录所需的一种蛋白质因子，已被纯化至近乎均一。TFIIF是由30 kDa和78 kDa多肽组成的异源二聚体。30 kDa和78 kDa多肽对于重建TFIIF活性都是必需的。TFIIF活性从凝胶过滤柱上洗脱时，表观分子量为220 kDa，这表明天然因子以异源二聚体的二聚体形式存在。

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