哺乳动物RNA聚合酶II起始转录所需的两种转录因子的纯化。
Purification of two transcription factors required for initiation by mammalian RNA polymerase II.
作者信息
Kawaguchi T, Kitajima S, Niho Y, Oda T, Germino J, Weissman S M, Yasukochi Y
机构信息
Department of Human Genetics, Medical Research Institute, Tokyo Medical and Dental University, Japan.
出版信息
Proc Natl Acad Sci U S A. 1990 Sep;87(17):6619-23. doi: 10.1073/pnas.87.17.6619.
We have purified two general transcription factors (FA and FE) necessary for specific transcription by mammalian RNA polymerase II to near homogeneity. Both activities are associated with peptides of approximately 33 kDa. FA and FE do not replace one another and show different kinetics of action in a sarkosyl block assay. In particular, FE participated in a rapid reaction after the formation of an initial complex with the other transcription factors. Furthermore, FE can associate with purified calf thymus RNA polymerase II.
我们已经将哺乳动物RNA聚合酶II进行特异性转录所必需的两种通用转录因子（FA和FE）纯化至近乎均一的状态。两种活性都与大约33 kDa的肽相关。FA和FE不能相互替代，并且在 Sarkosyl 阻断试验中表现出不同的作用动力学。特别是，FE在与其他转录因子形成初始复合物后参与了快速反应。此外，FE可以与纯化的小牛胸腺RNA聚合酶II结合。
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