Qin Liu-yan, Jiang Ni, Tang Mei-qiong, Miao Jian-hua, Li Lin-xuan
Guangxi Botanical Garden of Medicinal Plant, Nanning 530023, China.
Zhong Yao Cai. 2011 Apr;34(4):499-502.
To study the antagonism of Trichoderma spp. to fungi S9(Fusarium solani)which caused root rot of Sophora tonkinensis and discuss the further develop prospects of microbial biological control in soil-borne diseases on Chinese herbal medicines.
Antagonism of H2 (Trichoderma harsianum), M6 (Trichoderma viride) and K1 (Trichoderma koningii) to Fusarium solani were researched by growth rate and confront culture. And their mechanisms were discussed.
H2 and M6 had obvious competitive advantage, the growth rate of which were 1.43-2.72 times and 1.43-1.95 times as S9 respectively. The space competitive advantage of K1 was relatively weak; the growth rate was slower than S9. The antagonism of three species of Trichoderma spp. to S9 was in varying degrees. The antagonism to S9 of M6 and H2 was better,the inhibition rate were 100% and 82.35% respectively, even cultivated S9 for three days in advance. And their inhibition indexes were both reached class I. The inhibition index and inhibition rate of K1 was respectively 46.36% and class IV. The Trichoderma spp. could cause S9 mycelium to appear some phenomenon just like fracture, constriction reduced, digestion, etc. which were observed under the microscope.
Trichoderma harsianum and Trichoderma viride showed the further develop prospects in the fight against soil-borne disease on Chinese herbal medicines.
研究木霉菌对引起越南槐根腐病的茄腐镰刀菌的拮抗作用，探讨微生物生物防治在中药材土传病害防治中的进一步发展前景。
采用生长速率法和平板对峙培养法研究哈茨木霉H2、绿色木霉M6和康氏木霉K1对茄腐镰刀菌的拮抗作用，并探讨其作用机制。
H2和M6具有明显的竞争优势，其生长速率分别是S9的1.43 - 2.72倍和1.43 - 1.95倍。K1的空间竞争优势相对较弱，生长速率比S9慢。三种木霉菌对S9均有不同程度的拮抗作用。M6和H2对S9的拮抗效果较好，即使提前三天接种S9，其抑菌率仍分别达到100%和82.35%，抑菌指数均达到Ⅰ级。K1的抑菌指数和抑菌率分别为46.36%和Ⅳ级。显微镜下观察发现，木霉菌可使S9菌丝出现断裂、缢缩变细、消解等现象。
哈茨木霉和绿色木霉在防治中药材土传病害方面具有进一步的发展前景。
