Yu Li-ping, Wang Xiao-ke, Luo Jia-bo
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
Zhong Yao Cai. 2011 Apr;34(4):620-3.
To establish the method for determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride in maxingshigan decoction by capillary electrophoresis.
The separation was performed on a fused silica capillary of 60 cm x 55 microcrpm ID (52 cm of effective length). 60 mmol/L NaB4O7 + 10% (V/V) CH3OH (pH 9.0) was selected as the running buffer. The separation voltage was 12 kV. The samples was injected by gravity (10 s, 15 cm). The detection wavelength was 210 nm and berberine hydrochloride was the internal standard.
The linear range of determination for ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride were 20.0-160.0 microg/mL (r = 0.9999), 7.5-60.0 microg/mL (r = 0.9991) and 2.0-10.0 microg/mL (r = 0.9993). The average recoveries were 98.0%, 97.0% and 97.8%, the precisions of the method were 2.31%, 2.21% and 2.00% (n=6), respectively.
The method is convenient, rapid and accurate for the quality control of maxingshigan decoction.
建立毛细管电泳法测定麻杏石甘汤中盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱的方法。
采用内径55μm、有效长度52cm、总长60cm的熔融石英毛细管进行分离。选用60mmol/L硼砂+10%（V/V）甲醇（pH 9.0）作为运行缓冲液。分离电压为12kV。样品采用重力进样（10s，15cm）。检测波长为210nm，内标为盐酸小檗碱。
盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱的线性测定范围分别为20.0 - 160.0μg/mL（r = 0.9999）、7.5 - 60.0μg/mL（r = 0.9991）和2.0 - 10.0μg/mL（r = 0.9993）。平均回收率分别为98.0%、97.0%和97.8%，方法精密度分别为2.31%、2.21%和2.00%（n = 6）。
该方法用于麻杏石甘汤的质量控制简便、快速、准确。
