高效毛细管电泳法测定红花中腺苷、芦丁和槲皮素的含量
[Determination of adenosine, rutin and quercetin in Carthamus tinctorius by HPCE].
作者信息
Sun Yi, Guo Tao, Sui Yin, Li Fa-mei
机构信息
College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
出版信息
Yao Xue Xue Bao. 2003 Apr;38(4):283-5.
AIM
To develop a method for determination of adenosine, rutin and quercetin in Carthamus tinctorius L. by high performance capillary electrophoresis(HPCE).
METHODS
A fused silica capillary (66.5 cm x 50 microns ID, an effective length of 58 cm) was used. The running buffer composed of 50 mmol.L-1 borax (pH 9.7) containing 18% methanol. The applied voltage was 24 kV and the capillary temperature was 20 degrees C. The detection wavelength was 210 nm. Rifampicin was used as internal standard.
RESULTS
A good linearity between peak area ratio of the common peak to the internal standard and the concentration was found in the range of 10-160 mg.L-1 for adenosine, 100-2,000 mg.L-1 for rutin and 100-1,600 mg.L-1 for quercetin (r > 0.998). The average recoveries were 98.5%-100.5%, 96.9%-99.5% and 99.1%-99.5% for adenosine, rutin and quercetin, respectively. The relative standard deviation was less than 6.5% (n = 5).
CONCLUSION
The method is simple, rapid and with satisfactory recoveries and good reproducibilities. It can be used to control the quality of Carthamus tinctorius.
目的
建立高效毛细管电泳法测定红花中腺苷、芦丁和槲皮素的含量。
方法
采用熔融石英毛细管（66.5 cm×50 μm内径，有效长度58 cm）。运行缓冲液由含18%甲醇的50 mmol·L-1硼砂（pH 9.7）组成。施加电压为24 kV，毛细管温度为20℃。检测波长为210 nm。以利福平为内标。
结果
腺苷在10 - 160 mg·L-1、芦丁在100 - 2000 mg·L-1、槲皮素在100 - 1600 mg·L-1范围内，其与内标物的峰面积比和浓度呈良好的线性关系（r＞0.998）。腺苷、芦丁和槲皮素的平均回收率分别为98.5% - 100.5%、96.9% - 99.5%和99.1% - 99.5%。相对标准偏差小于6.˙5%（n = 5）。
结论
该方法简便、快速，回收率满意，重现性好。可用于红花的质量控制。
Zotero 插件