Livins'ka O P, Kovalenko N K, Harmasheva I L
Mikrobiol Z. 2011 May-Jun;73(3):26-32.
The methods of Gram-positive cells disintegration to obtain cell wall fragments have been described. It has been shown that the use of ultrasonic disintegrator UZD-500 at the frequency 22 kHz during 30 min is optimal for disintegration of lactic acid bacteria cells. Also the ability to use grinding of cells in liquid nitrogen (6 times to the complete evaporation) for obtaining the cell wall fragments has been demonstrated.
已描述了革兰氏阳性菌细胞裂解以获得细胞壁片段的方法。结果表明，使用频率为22kHz的超声破碎仪UZD-500处理30分钟，对乳酸菌细胞裂解最为适宜。此外，还证明了可通过在液氮中研磨细胞（完全蒸发6次）来获得细胞壁片段。
