Instituto de Investigaciones para la Industria Química (INIQUI-CONICET), A4402FDC- Salta, Salta, Argentina.
Microbiol Res. 2011 Jan 20;166(1):1-13. doi: 10.1016/j.micres.2010.01.003. Epub 2010 Jan 29.
Eight strains belonging to Lactobacillus spp. and five to Enterococcus spp. were isolated from the gut of worker Apis mellifera L. bees. Studies based on 16S rRNA sequencing revealed that AJ5, IG9, A15 and CRL1647 strains had a 99% identity with Lactobacillus johnsonii, while SM21 showed a 99% similarity with Enterococcus faecium. L. johnsonii CRL1647, AJ5 and IG9 were high lactic acid producers (values were between 177 and 275 mM), and in vitro they inhibited different human food-borne pathogens and Paenibacillus larvae, the American foulbrood agent. This bacterium was the most sensitive to the lactic acid effect being inhibited by 44 mM of this metabolite. L. johnsonii CRL1647, AJ5 and IG9 also presented important surface properties. These cells showed between 77% and 93% of auto-aggregation. The preliminary study of the chemical nature of the aggregating factors revealed that the molecules involved in the surface of each L. johnsonii strain were quite complex; and something of a peptidic nature was mainly involved. E. faecium SM21 produced bacteriocin-like compounds with anti-Listeria effects. Furthermore, a band close to 6.0-7.5 kDA was detected by SDS-PAGE studies, and the entA, B and P structural genes were amplified by PCR reactions. For the first time, bee-gut associated L. johnsonii and E. faecium strains have been isolated, identified, cultivated and some of their functional properties reported.
从工蜂肠道中分离出 8 株属于乳杆菌属和 5 株属于肠球菌属的菌株。基于 16S rRNA 测序的研究表明，AJ5、IG9、A15 和 CRL1647 株与 Johnson 乳杆菌具有 99%的同源性，而 SM21 与屎肠球菌具有 99%的相似性。L. johnsonii CRL1647、AJ5 和 IG9 是高乳酸生产菌（值在 177 至 275 mM 之间），并且在体外它们抑制了不同的人类食源性病原菌和美洲幼虫腐臭病菌，即美国幼虫腐臭病的病原体。这种细菌对这种代谢物抑制的乳酸效应最敏感，被 44mM 的乳酸抑制。L. johnsonii CRL1647、AJ5 和 IG9 还具有重要的表面特性。这些细胞的自动聚集率在 77%至 93%之间。对聚集因子化学性质的初步研究表明，每个 L. johnsonii 菌株表面涉及的分子非常复杂；并且主要涉及一些肽性质的分子。E. faecium SM21 产生具有抗李斯特菌作用的细菌素样化合物。此外，通过 SDS-PAGE 研究检测到接近 6.0-7.5kDa 的条带，并通过 PCR 反应扩增了 entA、B 和 P 结构基因。首次从蜜蜂肠道中分离、鉴定、培养并报告了一些功能特性的 L. johnsonii 和 E. faecium 菌株。
