Suppr超能文献

明胶微球中溶菌酶的体外释放:交联剂和热可逆凝胶作为悬浮介质的影响。

In vitro release of lysozyme from gelatin microspheres: effect of cross-linking agents and thermoreversible gel as suspending medium.

机构信息

Dipartimento di Scienze Chimiche, Università di Cagliari, CNBS and CSGI, Cittadella Universitaria, s.s. 554 bivio Sestu, 09042 Monserrato (CA), Italy.

出版信息

Biomacromolecules. 2011 Sep 12;12(9):3186-93. doi: 10.1021/bm200679w. Epub 2011 Aug 11.

Abstract

This study was aimed to characterize the microstructure and the performance of gelatin microspheres (GMs) cross-linked by two different cross-linkers viz. d-glucose and glutaraldehyde. New formulations were obtained, suspending the GMs in a thermoreversible Pluronic F127 (PF127) liquid-crystalline gel. Lysozyme was used as a model biomacromolecular drug to evaluate release features. Both types of cross-linked GMs were prepared by thermal gelation method. The lysozyme-loaded microspheres were characterized by scanning electron microscopy (SEM) for size distribution, shape, and surface texture. SEM revealed that both types of lysozyme-loaded GMs were spherical in shape and that the surface of glutaraldehyde cross-linked GMs was smoother than that of the glucose cross-linked GMs. The degree of cross-linking of microspheres was investigated using ATR-FTIR technique. The prepared GMs were suspended in 20% w/v aqueous PF127 gel for which the usual sol-gel transition temperature of 22 °C did not change in the presence of GMs, as indicated by rheological measurements. SAXS study of the PF127 gel confirmed the occurrence of a discrete cubic liquid-crystalline phase of the Fm3m type whose lattice parameter slightly decreased as a result of GMs addition. The in vitro release of lysozyme from both types of cross-linked GMs was successfully controlled when they were suspended in PF127 gel, thus suggesting the potential use of this new combined formulation as a drug-depot system.

摘要

本研究旨在表征由两种不同交联剂(即葡萄糖和戊二醛)交联的明胶微球(GMs)的微观结构和性能。通过悬浮在热可逆 Pluronic F127(PF127)液晶凝胶中的 GMs 获得了新的配方。溶菌酶被用作模型生物大分子药物来评估释放特性。两种类型的交联 GMs 均通过热凝胶化方法制备。用扫描电子显微镜(SEM)对载溶菌酶的微球进行了大小分布、形状和表面纹理的特征描述。SEM 表明,两种类型的载溶菌酶的 GMs 均为球形,戊二醛交联 GMs 的表面比葡萄糖交联 GMs 的表面更光滑。使用 ATR-FTIR 技术研究了微球的交联程度。制备的 GMs 悬浮在 20% w/v 的 PF127 水凝胶中,流变测量表明,即使存在 GMs,凝胶的通常溶胶-凝胶转变温度 22°C 也没有改变。SAXS 研究证实了 Fm3m 型离散立方液晶相的存在,其晶格参数由于 GMs 的加入而略有减小。当两种类型的交联 GMs 悬浮在 PF127 凝胶中时,溶菌酶的体外释放得到了成功控制,因此表明这种新的组合配方具有作为药物储存系统的潜力。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验