Liu Zhengwei, Tang Wanze, Liu Jiayi, Han Yingying, Yan Qinnan, Dong Yuechao, Liu Xiaomei, Yang Dazhi, Ma Guixing, Cao Huiling
Department of Biochemistry, School of Medicine, Southern University of Science and Technology, Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research, Shenzhen Key Laboratory of Cell Microenvironment, Shenzhen, 518055, China.
Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.
Bioact Mater. 2022 Jul 1;20:610-626. doi: 10.1016/j.bioactmat.2022.06.008. eCollection 2023 Feb.
A novel sprayable adhesive is established (ZnMet-PF127) by the combination of a thermosensitive hydrogel (Pluronic F127, PF127) and a coordination complex of zinc and metformin (ZnMet). Here we demonstrate that ZnMet-PF127 potently promotes the healing of traumatic skin defect and burn skin injury by promoting cell proliferation, angiogenesis, collagen formation. Furthermore, we find that ZnMet could inhibit reactive oxygen species (ROS) production through activation of autophagy, thereby protecting cell from oxidative stress induced damage and promoting healing of skin wound. ZnMet complex exerts better effects on promoting skin wound healing than ZnCl or metformin alone. ZnMet complex also displays excellent antibacterial activity against or which could reduce the incidence of skin wound infections. Collectively, we demonstrate that sprayable PF127 could be used as a new drug delivery system for treatment of skin injury. The advantages of this sprayable system are obvious: (1) It is convenient to use; (2) The hydrogel can cover irregular skin defect sites evenly in a liquid state. In combination with this system, we establish a novel sprayable adhesive (ZnMet-PF127) and demonstrate that it is a potential clinical treatment for traumatic skin defect and burn skin injury.
通过热敏水凝胶（普朗尼克F127，PF127）与锌和二甲双胍的配位络合物（ZnMet）相结合，制备了一种新型可喷涂粘合剂（ZnMet-PF127）。在此，我们证明ZnMet-PF127通过促进细胞增殖、血管生成、胶原蛋白形成，有力地促进创伤性皮肤缺损和烧伤皮肤损伤的愈合。此外，我们发现ZnMet可通过激活自噬抑制活性氧（ROS）的产生，从而保护细胞免受氧化应激诱导的损伤并促进皮肤伤口愈合。ZnMet络合物在促进皮肤伤口愈合方面比单独的ZnCl或二甲双胍具有更好的效果。ZnMet络合物还对金黄色葡萄球菌或大肠杆菌显示出优异的抗菌活性，这可以降低皮肤伤口感染的发生率。总体而言，我们证明可喷涂的PF127可作为治疗皮肤损伤的新型药物递送系统。这种可喷涂系统的优点显而易见：（1）使用方便；（2）水凝胶可以液态均匀覆盖不规则的皮肤缺损部位。结合该系统，我们制备了一种新型可喷涂粘合剂（ZnMet-PF127），并证明它是创伤性皮肤缺损和烧伤皮肤损伤的潜在临床治疗方法。
