Laboratoire de Chimie biologique et médicale et de Microbiologie pharmaceutique, Institut de Pharmacie Université libre de Bruxelles, Brussels, Belgium.
Biofouling. 2011 Sep;27(8):811-21. doi: 10.1080/08927014.2011.604776.
A study on biofilm formation was carried out using five methicillin-sensitive [MSSA] and five methicillin-resistant [MRSA] strains of S. aureus. In each group, there were four strains isolated from patients from Kinshasa (Democratic Republic of Congo, DRC) and one reference strain. All of the strains were hydrophobic. The adherence of the bacteria to an abiotic surface was studied with the Biofilm Ring Test (BFRT®) and the crystal violet staining method (CVSM). Both techniques showed that eight of the strains formed biofilms within 2-3 h. The extent of the biofilm formed by one strain could only be observed with the CVSM. Periodate prevented the formation of biofilms and, in separate experiments, destroyed the biofilm pre-formed by the MSSA reference, but not those pre-formed by the clinical strains. Proteinase K destroyed all pre-formed biofilms. Six of the strains were icaA+; the clinical MSSA strains were not. The results also indicated different mechanisms of biofilm development between MSSA and MRSA clinical strains. The BFRT® and the CVSM are complementary techniques to study the adhesion of bacteria and the development of biofilms.
采用 5 株耐甲氧西林金黄色葡萄球菌（MRSA）和 5 株甲氧西林敏感金黄色葡萄球菌（MSSA）对生物膜形成进行了研究。每组 4 株菌均来自金沙萨（刚果民主共和国）的患者，1 株为参考株。所有菌株均具有疏水性。采用生物膜环试验（BFRT®）和结晶紫染色法（CVSM）研究了细菌对非生物表面的黏附作用。这两种技术均表明，有 8 株菌在 2-3 小时内形成了生物膜。只有 CVSM 才能观察到 1 株菌形成的生物膜的程度。过碘酸盐可阻止生物膜的形成，在单独的实验中，过碘酸盐破坏了 MSSA 参考株预先形成的生物膜，但未破坏临床分离株预先形成的生物膜。蛋白酶 K 破坏了所有预先形成的生物膜。6 株菌为 icaA+，临床 MSSA 菌株则不是。结果还表明，MSSA 和 MRSA 临床分离株的生物膜发育机制不同。BFRT®和 CVSM 是研究细菌黏附和生物膜发育的互补技术。
