Department for Dermatology, Venerology and Allergology, University Hospital of Essen-Duisburg, Essen, GermanyInstitute for Microbiology, University Hospital of Essen-Duisburg, Essen, Germany.
Int Wound J. 2014 Feb;11(1):44-9. doi: 10.1111/j.1742-481X.2012.01036.x. Epub 2012 Jul 9.
Most chronic wounds are colonised with different microorganisms, especially problematic bacteria like methicillin-resistant Staphylococcus aureus (MRSA), which represent an increasing therapeutic challenge in the modern wound therapy regimen. Therefore, it is essential to specify the bacteria in wounds for an individual-specific treatment. In most patients, an exemplary bacterial swab is taken from the centre of the wound surface. This so-called Levine technique is propagated currently as the gold standard. The aim of our clinical investigation was to compare the results of different swab techniques to the new established Essen Rotary. In this monocentric prospective investigation, 50 patients with chronic leg ulcers were examined consecutively. The results of our clinical study show that bacteria are heterogeneously spread on wound surfaces. The analysis of the semiquantitative measured results showed that the Essen Rotary could detect significant more bacteria with a total amount of 111 bacteria (P = 0·049) compared to usual swab techniques. Considerably, only the Essen Rotary identified five compared to three MRSA-patients detected by other techniques. The Essen Rotary is an efficient, economic and uncomplicated modification of bacteriological swab techniques which detects significant more bacteria compared to other conventional swab techniques. Therefore, the Essen Rotary may become the new gold standard in routinely taken bacteriological swabs especially for MRSA screenings in patients with chronic leg ulcers.
大多数慢性伤口都被不同的微生物定植,尤其是耐甲氧西林金黄色葡萄球菌(MRSA)等有问题的细菌,这对现代伤口治疗方案构成了越来越大的治疗挑战。因此,为了进行个体化治疗,必须确定伤口中的细菌。在大多数患者中,会从伤口表面的中心采集典型的细菌拭子。这种所谓的 Levine 技术目前被宣传为金标准。我们的临床研究旨在比较不同拭子技术与新建立的 Essen Rotary 的结果。在这项单中心前瞻性研究中,连续检查了 50 名患有慢性腿部溃疡的患者。我们的临床研究结果表明,细菌在伤口表面呈异质分布。半定量测量结果分析表明,与常规拭子技术相比,Essen Rotary 可以检测到明显更多的细菌,总共有 111 种细菌(P=0.049)。值得注意的是,只有 Essen Rotary 鉴定出了 5 名而非其他技术鉴定出的 3 名 MRSA 患者。Essen Rotary 是一种高效、经济且简单的细菌拭子技术改良,与其他常规拭子技术相比,它可以检测到明显更多的细菌。因此,Essen Rotary 可能成为常规细菌拭子的新标准,特别是在慢性腿部溃疡患者中进行 MRSA 筛查时。