Inserm U1016, 75014 Paris, France.
Biol Cell. 2011 Nov;103(11):531-42. doi: 10.1042/BC20100112.
The identification of a source of stem cells able to regenerate skeletal muscle was the goal of numerous studies with the aim to develop new therapeutic approaches for genetic muscle diseases or muscle injuries. A series of studies have demonstrated that stem cells derived from various tissues may have a role in the regeneration of damaged muscles, but this contribution is always very weak. Thus we established a project aiming to reprogramme non-muscle cells into the skeletal striated differentiation pathway.
We transduced several human primary adult stem or progenitor cells using a recombinant lentivirus containing the coding sequence of the Myf5 gene considered as a master gene for the determination of skeletal striated muscle. These original results are the first demonstration of a myogenic conversion of human mesenchymal and endothelial cells by Myf5.
The procedure described in the present paper could be used to develop new research protocols with the prospect of using these cells as therapeutic agents.
能够再生骨骼肌的干细胞的鉴定是许多研究的目标，这些研究旨在为遗传性肌肉疾病或肌肉损伤开发新的治疗方法。一系列研究表明，源自各种组织的干细胞可能在受损肌肉的再生中发挥作用，但这种贡献总是非常微弱。因此，我们开展了一个项目，旨在将非肌肉细胞重编程为骨骼肌条纹分化途径。
我们使用含有 Myf5 基因编码序列的重组慢病毒转导了几种人类原代成体干细胞或祖细胞，Myf5 被认为是决定骨骼肌条纹的主基因。这些原始结果首次证明了 Myf5 对人类间充质和内皮细胞的成肌转化。
本文所述的程序可用于开发新的研究方案，有望将这些细胞用作治疗剂。
