Laboratory of Animal Genetics and Breeding, Department of Animal Science, Tianjin Agricultural University, Tianjin, 300384, P. R. China.
Cell Biol Int. 2013 Sep;37(9):972-6. doi: 10.1002/cbin.10124. Epub 2013 May 23.
The formation of vertebrate skeletal muscles widely thought to be under the control of hierarchy of regulatory genes. MYF5 is one of the myogenic determination gene expressed in the developing mouse dermomyotome which control skeletal muscle differentiation. In the current work, we had obtained the cDNA sequence including the full coding region of the bos taurus myogenic factor MYF5 by reverse transcription polymerase chain reaction. Furthermore, we examined whether fibroblast cell derived from mouse and bos taurus can be transduced using plasmid vectors carrying bos taurus MYF5. Bos taurus MYF5 activates MYF5 and MYOD1 expression after 1 day culture. The concerted upregulation of the myogenic regulatory factors enhanced myosin (skeletal fast) expression. These observation show that MYF5 is essential for myogenic differentiation and provides candidates for regulation bos taurus skeletal muscle development.
脊椎动物骨骼肌的形成被广泛认为是受一系列调节基因控制的。在发育中的鼠真皮肌节中表达的肌生成决定基因 MYF5 就是控制骨骼肌分化的基因之一。在本研究中，我们通过反转录聚合酶链反应获得了包括牛肌生成因子 MYF5 全长编码区的 cDNA 序列。此外，我们还研究了携带牛 MYF5 的质粒载体是否可以转导来自鼠和牛的成纤维细胞。牛 MYF5 在培养 1 天后可激活 MYF5 和 MYOD1 的表达。肌调节因子的协同上调增强了肌球蛋白（骨骼肌快）的表达。这些观察结果表明，MYF5 对成肌分化是必需的，并为牛骨骼肌发育的调控提供了候选基因。
