Chen L C, Borges T, Glauert H P, Knight S A, Sunde R A, Schramm H, Oesch F, Chow C K, Robertson L W
Graduate Center for Toxicology, University of Kentucky, Lexington 40506-0054.
J Nutr. 1990 Mar;120(3):298-304. doi: 10.1093/jn/120.3.298.
Forty-eight male Sprague-Dawley rats fed a diet containing 0.4, 0.2 or 1.0 mg of selenium (Se)/kg of diet were injected with a single dose (35 mg/kg) of perfluorodecanoic acid (PFDA) in corn oil and killed 2 wk later. Control animals were pair-fed and treated with an equal volume of vehicle. PFDA treatment significantly increased Se-dependent glutathione peroxidase (Se-GSHPx) activity in liver cytosol of rats fed the 0.04 mg of Se/kg of diet but not in rats fed the other diets. The increase in liver cytosolic Se-GSHPx activity in rats fed 0.04 mg of Se/kg of diet paralleled increases in Se content and serum Se-GSHPx activity. Determination of Se-GSHPx by an enzyme-linked immunosorbent assay showed that PFDA caused a decrease in Se-GSHPx protein in rats fed 0.2 or 1.0 mg of Se/kg of diet but not in rats fed 0.04 mg of Se/kg of diet. Further analysis revealed that the ratio of Se-GSHPx activity to antibody-reactive protein was increased by PFDA in all three groups. The in vitro addition of PFDA directly to the assay mixture for Se-GSHPx activity did not produce any effect. Reduced glutathione was significantly increased by PFDA treatment in all three groups. These data show that PFDA affects the Se content, Se-GSHPx activity and Se-GSHPx protein in rat liver and that the effect is dependent on the dietary/hepatic Se level.
给48只雄性Sprague-Dawley大鼠喂食含0.4、0.2或1.0毫克硒（Se）/千克饲料的日粮，然后给它们单次注射（35毫克/千克）全氟癸酸（PFDA）的玉米油溶液，并在2周后处死。对照动物采用配对饲养，并给予等量的赋形剂。PFDA处理显著增加了日粮中硒含量为0.04毫克/千克的大鼠肝胞液中硒依赖性谷胱甘肽过氧化物酶（Se-GSHPx）的活性，但对日粮中硒含量为其他水平的大鼠没有影响。日粮中硒含量为0.04毫克/千克的大鼠肝胞液中Se-GSHPx活性的增加与硒含量和血清Se-GSHPx活性的增加平行。通过酶联免疫吸附测定法测定Se-GSHPx发现，PFDA导致日粮中硒含量为0.2或1.0毫克/千克的大鼠中Se-GSHPx蛋白减少，但对日粮中硒含量为0.04毫克/千克的大鼠没有影响。进一步分析表明，PFDA使所有三组中Se-GSHPx活性与抗体反应性蛋白的比率增加。在体外将PFDA直接添加到Se-GSHPx活性测定混合物中没有产生任何影响。PFDA处理使所有三组中的还原型谷胱甘肽显著增加。这些数据表明，PFDA影响大鼠肝脏中的硒含量、Se-GSHPx活性和Se-GSHPx蛋白，且这种影响取决于日粮/肝脏中的硒水平。
