Kelling C K, Van Rafelghem M J, Menahan L A, Peterson R E
Biochem Pharmacol. 1987 Apr 15;36(8):1337-44. doi: 10.1016/0006-2952(87)90091-8.
Perfluorodecanoic acid (PFDA) alters the circulating level of thyroid hormones, but the physiological significance of this change at the target tissue remains to be defined. To this end, the activities of thyroid-responsive hepatic enzymes were examined in adult male rats 1 week after treatment with a single dose of PFDA (20, 40 or 80 mg/kg). Since PFDA treatment caused a dose-related reduction in feed intake, vehicle-treated rats pair-fed to their counterparts receiving PFDA were used to determine if any of the PFDA-induced alterations in enzyme activity were secondary to hypophagia. Following the administration of PFDA, L-glycerol-3-phosphate dehydrogenase, a liver mitochondrial enzyme sensitive to thyroid status, exhibited a modest increase in activity, whereas that of succinate dehydrogenase, a constitutive mitochondrial marker enzyme, was similar in both PFDA-treated rats and their pair-fed counterparts at all dose levels examined. Activity of cytosolic lactate dehydrogenase was also augmented modestly in livers of rats receiving PFDA. In contrast, activity of cytosolic malic enzyme, a thyroid-responsive enzyme, was increased markedly in PFDA-treated rats. Hepatic activity of glucose-6-phosphate dehydrogenase, which also responds to alterations in thyroid status, exhibited a modest increase with 20 and 40 mg/kg PFDA but was similar in both PFDA-treated rats and their pair-fed counterparts at the 80 mg/kg dose level. Absolute and relative liver mass was elevated in PFDA-treated rats at all dose levels in comparison to the appropriate vehicle-treated pair-fed animals. Total hepatic content of DNA was maintained in PFDA-treated rats at all dose levels, whereas a significant decrease in liver DNA was found in the vehicle-treated rats pair-fed to animals receiving 80 mg/kg PFDA. Following administration of PFDA, protein content per total liver was similar to that of their pair-fed counterparts. Thus, the pattern of activity of thyroid-responsive hepatic enzymes was not compatible with a functional shift toward a lessened thyroid status in rats treated with PFDA.
全氟癸酸（PFDA）会改变甲状腺激素的循环水平，但这种变化在靶组织中的生理意义仍有待确定。为此，在成年雄性大鼠单次注射PFDA（20、40或80mg/kg）1周后，检测了甲状腺反应性肝酶的活性。由于PFDA处理导致进食量呈剂量相关减少，因此将接受溶剂处理并与接受PFDA的大鼠配对饲养的大鼠用于确定PFDA诱导的酶活性改变是否继发于摄食减少。注射PFDA后，对甲状腺状态敏感的肝线粒体酶L-3-磷酸甘油脱氢酶的活性适度增加，而组成型线粒体标记酶琥珀酸脱氢酶的活性在所有检测剂量水平的PFDA处理大鼠及其配对饲养的大鼠中相似。接受PFDA的大鼠肝脏中胞质乳酸脱氢酶的活性也适度增强。相比之下，甲状腺反应性酶胞质苹果酸酶的活性在PFDA处理的大鼠中显著增加。同样对甲状腺状态改变有反应的葡萄糖-6-磷酸脱氢酶的肝脏活性在PFDA剂量为20和40mg/kg时适度增加，但在80mg/kg剂量水平时，PFDA处理大鼠及其配对饲养的大鼠中相似。与相应的接受溶剂处理的配对饲养动物相比，所有剂量水平的PFDA处理大鼠的绝对和相对肝脏重量均升高。所有剂量水平的PFDA处理大鼠肝脏中的总DNA含量保持不变，而与接受80mg/kg PFDA的动物配对饲养的接受溶剂处理的大鼠肝脏DNA显著减少。注射PFDA后，每只大鼠肝脏的蛋白质含量与其配对饲养的大鼠相似。因此，PFDA处理大鼠中甲状腺反应性肝酶的活性模式与甲状腺功能向降低状态的功能性转变不相符。
