Department of Biomedical Engineering, Korea University, Seoul, Korea.
Anal Chem. 2011 Sep 15;83(18):7221-6. doi: 10.1021/ac200859b. Epub 2011 Aug 16.
A tunable process for polydimethylsiloxane (PDMS) nanoslit fabrication is developed for nanofluidic applications. A microcontact printing (μCP) of a laterally spreading self-assembled hexadecanethiol (HDT) layer, combined with in situ curing of a sliding SU-8 droplet, enables precise and independent tuning of a nanoslit-mold width and height using a single μCP master mold. The SU-8 nanoslit-mold is replicated using a hard-soft composite PDMS to prevent channel collapse at low (<0.2) aspect ratio (height over width). The fluidic characteristics as well as dimensions of nanoslits fabricated with various conditions are analyzed using a fluorescein sample and AFM images. Finally, concentration polarization-based sample preconcentration is successfully demonstrated at the nanoslit boundary where an electric double-layer is overlapped.
用于纳米流控应用的聚二甲基硅氧烷 (PDMS) 纳米缝可调工艺的开发。通过横向扩展的自组装十六烷硫醇 (HDT) 层的微接触印刷 (μCP)，结合滑动 SU-8 液滴的原位固化，使用单个 μCP 主模具可以精确且独立地调节纳米缝模具的宽度和高度。使用硬软复合 PDMS 复制 SU-8 纳米缝模具，以防止在低纵横比 (<0.2) 时通道塌陷。使用荧光素样品和 AFM 图像分析了在各种条件下制造的纳米缝的流体特性和尺寸。最后，在电双层重叠的纳米缝边界处成功地演示了基于浓差极化的样品预浓缩。
