Nishimura T, Goodnight R, Prendergast R A, Ryan S J
Department of Ophthalmology, University of Southern California, Los Angeles.
Ophthalmologica. 1990;200(1):39-44. doi: 10.1159/000310075.
Laser-induced subretinal neovascularization (SRN) in monkey retinas was investigated by immunohistochemical techniques to identify the presence and location of activated macrophages. Retinal lesions were examined 3, 8 and 14 days after intensive argon laser treatment, and the distribution of interleukin-1 (IL-1)-containing cells in the lesions was determined by the orthogonal reconstruction of serial sections. Macrophages were present in the subretinal space of day 3 and day 8 lesions. These IL-1-containing cells were distributed about the area of rupture of Bruch's membrane and were quite common in lesions taken 3 days following laser treatment. While still apparent, the number was decreased at 8 days, and none were found 14 days after laser treatment. The temporal and spatial distribution of IL-1-staining macrophages paralleled the development of SRN, suggesting a relationship between the presence of activated macrophages and the initiation of neovascularization in this model.
采用免疫组织化学技术研究猴视网膜激光诱导的视网膜下新生血管形成（SRN），以确定活化巨噬细胞的存在和位置。在氩激光密集治疗后3天、8天和14天检查视网膜病变，并通过连续切片的正交重建确定病变中含白细胞介素-1（IL-1）细胞的分布。巨噬细胞存在于第3天和第8天病变的视网膜下间隙。这些含IL-1的细胞分布在 Bruch 膜破裂区域周围，在激光治疗后3天的病变中相当常见。虽然仍然明显，但数量在8天时减少，在激光治疗后14天未发现。IL-1染色巨噬细胞的时间和空间分布与SRN的发展平行，表明在该模型中活化巨噬细胞的存在与新生血管形成的起始之间存在关联。
