Centre for Immune Regulation and Reproductive Immunology (CIRRI), Department of Clinical Biochemistry, Copenhagen University Hospital (Roskilde) and Roskilde Hospital, 7-13 Køgevej, DK-4000 Roskilde, Denmark.
Mol Hum Reprod. 2011 Dec;17(12):727-38. doi: 10.1093/molehr/gar052. Epub 2011 Aug 3.
One of the non-classical human leukocyte antigen (HLA) class Ib proteins, HLA-G, is believed to exert important immunoregulatory functions, especially during pregnancy. The presence of HLA protein in paternal seminal fluid has been suggested to have an influence on the risk of developing pre-eclampsia. We have investigated whether HLA-G protein is present in human seminal plasma and in different tissue samples of the male reproductive system. Western blot technique and a soluble HLA-G (sHLA-G) assay were used to detect sHLA-G in human seminal plasma samples. Immunohistochemical staining was performed on paraffin-embedded tissue samples. We detected sHLA-G protein in seminal plasma, and HLA-G expression in normal testis and in epididymal tissue of the male reproductive system but not in the seminal vesicle. Furthermore, the results indicated a weak expression of HLA-G in hyperplastic prostatic tissue. In summary, several of the findings reported in this study suggest an immunoregulatory role of HLA-G in the male reproductive system and in seminal plasma.
人类白细胞抗原（HLA）I 类 Ib 蛋白之一 HLA-G 被认为具有重要的免疫调节功能，尤其是在怀孕期间。有人提出，父系精液中 HLA 蛋白的存在可能会影响子痫前期的发病风险。我们研究了 HLA-G 蛋白是否存在于人类精液和男性生殖系统的不同组织样本中。Western blot 技术和可溶性 HLA-G（sHLA-G）检测用于检测人类精液样本中的 sHLA-G。免疫组织化学染色在石蜡包埋组织样本上进行。我们在精液中检测到 sHLA-G 蛋白，在男性生殖系统的正常睾丸和附睾组织中检测到 HLA-G 表达，但在精囊组织中未检测到。此外，结果表明 HLA-G 在前列腺增生组织中有微弱表达。总之，本研究的部分结果提示 HLA-G 在男性生殖系统和精液中具有免疫调节作用。
