Goldoni Matteo, Tagliaferri Sara
Department of Clinical Medicine, Nephrology, and Preventive Science, ISPELS Research Center, University of Parma Medical School, Parma, Italy.
Methods Mol Biol. 2011;758:415-34. doi: 10.1007/978-1-61779-170-3_28.
Interactions among neurotoxicants in in vitro models, where the molecular mechanisms of toxicity are generally studied, represent today an emerging field in the experimental neurotoxicology. In this chapter, we define some general concepts about the optimization of in vitro experiments to assess the dose/concentration-effect/response relationships and to extrapolate the functions describing them. After describing the available models to study interactions (the Bliss independence criterion and the Loewe additivity model), we present a method to practically apply these models to experimental data. Finally, we provide some examples of the theory of interactions among neurotoxicants in in vitro models.
在通常研究毒性分子机制的体外模型中，神经毒物之间的相互作用如今代表了实验神经毒理学中一个新兴的领域。在本章中，我们定义了一些关于优化体外实验的一般概念，以评估剂量/浓度-效应/反应关系，并推断描述这些关系的函数。在描述了用于研究相互作用的现有模型（布利斯独立性标准和洛伊相加模型）之后，我们提出了一种将这些模型实际应用于实验数据的方法。最后，我们提供了一些体外模型中神经毒物相互作用理论的实例。
