Bhagwat Medha, Bhagwat Arvind A
NIH Library, Office of Research Services, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2011;765:297-307. doi: 10.1007/978-1-61779-197-0_17.
Fully annotated genome sequences of many microorganisms are publicly available as a resource. However, in-depth analysis of these genomes using specialized tools is required to derive meaningful information. We describe here the utility of three powerful publicly available genome databases and analysis tools. Protocols outlined here are particularly useful for performing pairwise genome comparisons between closely related microorganisms to identify similarities and unique features, for example to identify genes specific to a pathogenic strain of Escherichia coli compared to a nonpathogenic strain.
许多微生物的全注释基因组序列作为一种资源已公开可用。然而，需要使用专门工具对这些基因组进行深入分析，以获取有意义的信息。我们在此描述三个强大的公开可用基因组数据库和分析工具的用途。这里概述的方案对于在密切相关的微生物之间进行成对基因组比较以识别相似性和独特特征特别有用，例如与非致病菌株相比，识别大肠杆菌致病菌株特有的基因。
