Bigović Dubravka, Savikin Katarina, Janković Teodora, Menković Nebojsa, Zdunić Gordana, Stanojković Tatjana, Djurić Zorica
Institute for Medicinal Plant Research "Dr Josif Pancić", Tadeusa Kosćuska 1, Belgrade, Serbia.
Nat Prod Commun. 2011 Jun;6(6):819-22.
Flowers of Helichrysum plicatum were extracted under different experimental conditions, and their antioxidant activity was determined by DPPH radical scavenging assay. Extracts obtained with higher concentration of ethyl acetate (90% or 100%) were found to contain the greatest amount of total phenolics (> 250 mg gallic acid equivalents/g of dried extract), and high correlation between total phenolic content and antiradical activity was observed (r = -0.79). Based on the total phenolic content and antiradical activity, some extracts were selected for investigation of cytotoxic activity toward PC3, HeLa and K562 human cancer cell lines in vitro. All tested extracts exhibited moderate activity against HeLa cells (41.9-42.1 microg/mL), whereas the extract obtained with 100% ethyl acetate was the most active against K562 and PC3 cell lines (25.9 and 39.2 microg/mL, respectively). Statistical analysis revealed significant correlation between total phenolic content and cytotoxic activity against PC3 and K562 cells. HPLC identification of phenolic compounds from the extracts indicated the presence of apigenin, naringenin and kaempferol as free aglycones, and glycosides of apigenin, naringenin, quercetin and kaempferol. Among aglycones, kaempferol displayed moderate cytostatic activity against all cell lines (24.8-64.7 microM).
在不同实验条件下提取了皱叶蜡菊的花朵，并通过DPPH自由基清除试验测定了其抗氧化活性。发现用较高浓度乙酸乙酯（90%或100%）获得的提取物含有最多的总酚类物质（>250毫克没食子酸当量/克干提取物），并且观察到总酚含量与抗自由基活性之间存在高度相关性（r = -0.79）。基于总酚含量和抗自由基活性，选择了一些提取物来研究其对PC3、HeLa和K562人癌细胞系的体外细胞毒性活性。所有测试提取物对HeLa细胞均表现出中等活性（41.9 - 42.1微克/毫升），而用100%乙酸乙酯获得的提取物对K562和PC3细胞系活性最高（分别为25.9和39.2微克/毫升）。统计分析表明总酚含量与对PC3和K562细胞的细胞毒性活性之间存在显著相关性。对提取物中酚类化合物的HPLC鉴定表明存在芹菜素、柚皮素和山奈酚作为游离苷元，以及芹菜素、柚皮素、槲皮素和山奈酚的糖苷。在苷元中，山奈酚对所有细胞系均表现出中等的细胞生长抑制活性（24.8 - 64.7微摩尔）。
