Department of Biology, Faculty of Sciences, Anadolu University, Eskişehir, Turkey.
J Enzyme Inhib Med Chem. 2012 Jun;27(3):458-66. doi: 10.3109/14756366.2011.596835. Epub 2011 Aug 4.
In the present study, Au(III) and Pt(II) complexes of 1, 10-phenanthroline (phen) were synthesized and used as the test compounds. The structure elucidation of the synthesized compounds was performed by IR, (1)H-NMR and MASS spectroscopic data and the results of elemental analyses. The cytotoxic and apoptotic effects of test compounds were elucidated on V79 379A (Chinese hamster lung fibroblast like) and A549 (human lung carcinoma epithelial like) cell lines. Cytotoxicity was measured with MTT assay and antitumoral effect was determined by colony forming ability methods. In addition, nuclear fragmentation and activation of apoptotic enzyme (caspase-3) and DAPI staining were used to detect the apoptotic effect of the compounds. All the test compounds induced time and concentration-dependent cytotoxic and antitumoral effects. Significant increases in the levels of apoptosis were observed with increasing exposure concentration.
在本研究中，我们合成了 1,10-菲啰啉（phen）的 Au(III) 和 Pt(II) 配合物，并将其用作测试化合物。通过 IR、(1)H-NMR 和 MASS 光谱数据以及元素分析的结果对合成化合物的结构进行了阐明。测试化合物的细胞毒性和细胞凋亡作用在 V79 379A（中国仓鼠肺成纤维样）和 A549（人肺癌上皮样）细胞系上进行了阐明。通过 MTT 测定法测量了细胞毒性，并通过集落形成能力方法确定了抗肿瘤作用。此外，核碎裂和凋亡酶（半胱天冬酶-3）的激活以及 DAPI 染色用于检测化合物的细胞凋亡作用。所有测试化合物均诱导了时间和浓度依赖性的细胞毒性和抗肿瘤作用。随着暴露浓度的增加，凋亡水平显著增加。
