Department of Dental Material, Azad University, Tehran, Iran.
Acta Odontol Scand. 2012 Dec;70(6):536-40. doi: 10.3109/00016357.2011.600715. Epub 2011 Aug 5.
Knowledge about the effect of storage conditions on the clinical performance of Mineral Trioxide Aggregate (MTA) is of great importance for clinicians. The aim of this study was to evaluate the effect of storage temperature on sealing ability and solubility of white MTA.
This study was divided into two parts: sealing ability and solubility tests. Forty single-rooted human pre-molars were divided into three experimental (n = 10) and two control groups (n = 5). The root canals were instrumented and root apices were resected. Root-end cavities were prepared and filled with white MTA stored at 4°C, 25°C or 40°C. Microleakage was evaluated using a protein leakage test with bovine serum albumin at 24-h intervals for 120 days. The solubility test was also performed based on ISO specifications (ISO 2001-6876). Data were analyzed by one-way analysis of variance and post-hoc Tukey test, with a significance level set at p = 0.05.
The highest bovine serum albumin microleakage and the highest solubility rates were observed in 4°C followed by 25°C and 40°C groups. At higher temperatures, leakage needed significantly longer times to occur (p < 0.05).
Based on these findings, storage temperature appears to play an important role in the properties and hence clinical outcomes of MTA.
了解储存条件对矿物三氧化物聚合体（MTA）临床性能的影响对临床医生非常重要。本研究旨在评估储存温度对白色 MTA 密封能力和溶解度的影响。
本研究分为两部分：密封能力和溶解度测试。将 40 颗单根人磨牙随机分为三组（n = 10）和两组对照（n = 5）。根管预备并切除根尖。制备根管末端腔并用白色 MTA 填充，分别储存于 4°C、25°C 或 40°C。在 120 天内，每 24 小时使用牛血清白蛋白进行蛋白质渗漏测试评估微渗漏。根据 ISO 规范（ISO 2001-6876）还进行了溶解度测试。采用单因素方差分析和事后 Tukey 检验进行数据分析，显著性水平设为 p = 0.05。
在 4°C 下观察到最高的牛血清白蛋白渗漏和最高的溶解度，其次是 25°C 和 40°C 组。在较高温度下，泄漏需要更长的时间才能发生（p < 0.05）。
根据这些发现，储存温度似乎对 MTA 的性能和临床结果有重要影响。
