Molekulare Pflanzenphysiologie, Department Biologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Plant Biol (Stuttg). 2011 Sep;13(5):725-30. doi: 10.1111/j.1438-8677.2010.00431.x. Epub 2011 Jan 28.
Plant U-box armadillo repeat (PUB-ARM) proteins represent a type of E3 ubiquitin ligase. PUB-ARM proteins have various functions in plant development, plant defence and plant stress responses. The so far uncharacterised PUB-ARM proteins AtPUB18 and AtPUB19 are highly homologous to each other and regulated on the transcript level by ABA and NaCl. To investigate their physiological function, we isolated and characterised two Arabidopsis mutants and named them pub18-1 and pub19-1. However, these single mutants did not show any ABA- or salt-dependent phenotype. In contrast, pub18-1/pub19-1 double mutant seeds were less sensitive to ABA and NaCl inhibition of seed germination compared to wild-type seeds, indicating that both PUB-ARM proteins may coordinately function as regulatory components in ABA or salt inhibition of germination.
植物 U-box 臂突重复（PUB-ARM）蛋白是一种 E3 泛素连接酶。PUB-ARM 蛋白在植物发育、植物防御和植物应激反应中具有多种功能。迄今为止尚未表征的 PUB-ARM 蛋白 AtPUB18 和 AtPUB19 彼此高度同源，并在转录水平上受到 ABA 和 NaCl 的调节。为了研究它们的生理功能，我们分离并表征了两个拟南芥突变体，并将它们命名为 pub18-1 和 pub19-1。然而，这些单突变体在 ABA 或盐依赖的表型上没有表现出任何差异。相比之下，pub18-1/pub19-1 双突变体种子对 ABA 和 NaCl 抑制种子萌发的敏感性低于野生型种子，表明这两种 PUB-ARM 蛋白可能作为协同作用的调节因子，参与 ABA 或盐抑制萌发的过程。
