Institute of Materials Science and Technology (IMT), Friedrich-Schiller-University Jena, Jena, Germany.
Dent Mater. 2011 Oct;27(10):1017-23. doi: 10.1016/j.dental.2011.07.001. Epub 2011 Aug 3.
The consumption of acidic soft drinks may lead to demineralization and softening of human dental enamel, known as dental erosion. The aims of this in vitro study were to determine: (i) if different acids with a similar sensorial acidic taste lead to different hardness loss of enamel and (ii) if the fruit acids tartaric, malic, lactic or ascorbic acid lead to less hardness loss of enamel than citric or phosphoric acid when their concentration in solution is based on an equivalent sensorial acidic taste.
Enamel samples of non-erupted human third molars were treated with acidic solutions of tartaric (TA), malic (MA), lactic (LA), ascorbic (AA), phosphoric (PA) and citric (CA) acids with a concentration that gave an equivalent sensorial acidic taste. The acidic solutions were characterized by pH value and titratable acidity. Atomic force microscopy (AFM) based nanoindentation was used to study the nano mechanical properties and scanning electron microscopy (SEM) was used to study the morphology of the treated enamel samples and the untreated control areas, respectively.
The investigated acids fell into two groups. The nano hardnesses of MA, TA and CA treated enamel samples (group I) were statistically significantly greater (p<0.05) than the nano hardnesses of PA, AA and LA treated enamel samples (group II). Within each group the nano hardness was not statistically significantly different (p>0.05). The SEM micrographs showed different etch prism morphologies depending on the acid used.
In vitro, the acids investigated led to different erosion effects on human dental enamel, despite their equivalent sensorial acidic taste. This has not been reported previously.
酸性软饮料的摄入可能导致人类牙釉质脱矿和软化，即牙侵蚀。本体外研究旨在确定：（i）具有相似感官酸性味道的不同酸是否会导致牙釉质硬度损失不同，以及（ii）如果基于等效感官酸性味道，将溶液中的浓度设定为酒石酸、苹果酸、乳酸或抗坏血酸，则水果酸酒石酸、苹果酸、乳酸或抗坏血酸是否会导致牙釉质硬度损失小于柠檬酸或磷酸。
用具有等效感官酸性味道的浓度处理未萌出的人第三磨牙的牙釉质样本，处理用的酸性溶液有酒石酸（TA）、苹果酸（MA）、乳酸（LA）、抗坏血酸（AA）、磷酸（PA）和柠檬酸（CA）。将酸性溶液的 pH 值和可滴定酸度进行了特征描述。基于原子力显微镜（AFM）的纳米压痕用于研究纳米力学性能，扫描电子显微镜（SEM）分别用于研究处理后的牙釉质样本和未经处理的对照区域的形态。
所研究的酸分为两组。经 MA、TA 和 CA 处理的牙釉质样本的纳米硬度（组 I）明显大于（p<0.05）经 PA、AA 和 LA 处理的牙釉质样本的纳米硬度（组 II）。在每组内，纳米硬度没有明显差异（p>0.05）。SEM 显微照片显示，根据使用的酸，蚀变棱锥形态不同。
在体外，尽管具有等效感官酸性味道，但所研究的酸对人类牙釉质的侵蚀效果不同。这以前没有报道过。
